The evolution of ATPase activity in SMC proteins

N Cobbe, M M S Heck

Research output: Contribution to journalArticlepeer-review

Abstract

The SMC (structural maintenance of chromosomes) proteins are a highly conserved and ubiquitous family of ATPases, found in nearly all living organisms examined, where they play crucial roles in transmission of the hereditary material. However, the extent to which efficient ATP hydrolysis is required for SMC function has been a matter of some debate. Here we investigate the potential functional significance of ATP binding and hydrolysis in different eukaryotic SMC proteins, both by comparing the conservation of conserved ATPase motifs and by exploring potential coevolution between associated domains. In this way, we have been able to account for the reduced requirement for ATPase activity in cohesin's SMC3 and demonstrate the greater apparent conservation requirements for such activity in condensin SMC proteins. Finally, we explore possible interactions between the SMC and non-SMC components of the condensin complex that are required for full condensin activity and may modulate ATPase activity in the holocomplex.

Original languageEnglish
Pages (from-to)685-696
Number of pages12
JournalProteins: Structure, Function, and Bioinformatics
Volume63
Issue number3
DOIs
Publication statusPublished - 15 May 2006

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