TY - BOOK
T1 - The Ex Vivo Organ Culture of Bone
AU - Staines, Katherine A
AU - Brown, Genevieve
AU - Farquharson, Colin
PY - 2019/2/8
Y1 - 2019/2/8
N2 - The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).
AB - The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).
UR - https://link.springer.com/bookseries/7651
U2 - 10.1007/978-1-4939-8997-3_10
DO - 10.1007/978-1-4939-8997-3_10
M3 - Book
C2 - 30729466
VL - 1914
T3 - Methods in Molecular Biology
BT - The Ex Vivo Organ Culture of Bone
PB - Humana Press
ER -