The firA gene, a locus involved in the expression of rifampicin resistance in Escherichia coli - II. Characterisation of bacterial proteins coded by λfirA transducing phages

R. Lathe*, J. P. Lecocq

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The firA gene probably codes for an essential component of the transcription machinery in E. coli. Bacterial proteins coded by λfirA transducing phages have been examined after infection of a UV-irradiated λ lysogen, and 2 major fir-specific proteins have been characterized. The larger protein has a molecular weight of 27,000 daltons. The smaller protein, of 17,000 daltons, is produced in a considerable molar excess over the larger protein, is basic and binds strongly to DNA-, to DEAE-and to phospho-cellulose. This protein is clearly visible upon 2-dimensional gel electrophoresis of unfractionated E. coli protein, showing that it is present in the cell in large quantities. Evidence is presented to suggest that this protein may be identical to the Kappa factor of Schäfer and Zillig (1973).

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalMolecular and General Genetics MGG
Volume154
Issue number1
DOIs
Publication statusPublished - Jan 1977

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