The firA gene, a locus involved in the expression of rifampicin resistance in Escherichia coli - I. Characterisation of λfirA transducing phages constructed in vitro

R. Lathe*, J. P. Lecocq, A. Resibois

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The firA200 mutation of E. coli not only renders RNA synthesis thermosensitive but also eliminates the high-level resistance to rifampicin associated with certain mutations in the β subunit of the RNA polymerase. A priori, the firA gene is likely to code for an essential component of the transcription apparatus. The isolation is reported of transducing phages for the firA gene, constructed in vitro by fusing fragments of the E. coli chromosome into a lambdoid bacteriophage. Such phages carry at least two essential genes and are able to suppress both the thermosensitivity and abnormal rifampicin sensitivity associated with the firA200 allele. The finding that some, but not all, of the λfirA phages have a temperature dependent growth defect is discussed.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalMolecular and General Genetics MGG
Volume154
Issue number1
DOIs
Publication statusPublished - Jan 1977

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