The Function of TALPID3

Research output: Contribution to conferenceAbstract

Abstract

TALPID3 (KIAA0586) is an essential gene for vertebrate development. TALPID3 was first mapped in the recessive, embryonic lethal, polydactylous talpid3 chicken breed at The Roslin Institute, Edinburgh (Davey et al. 2006). The TALPID3 protein localises to the centrosome in human, chicken mouse and zebrafish cells. A loss of TALPID3 function in mouse and chicken causes a failure of centrosome migration/orientation to the cell membrane resulting in a loss primary and motile cilia and related PCP and Hedgehog signalling defects. TALPID3 is a long protein with little homology to other proteins other than coiled coil domains. Characterisation by complementation of mutant chicken embryos with deletion constructs targeting conserved regions, has determined two regions essential for function, which encode coiled coil domain ‘4’ and a region predicted to contain a proline rich disordered domain. In order to investigate potential binding partners of TALPID3 we have undertaken a Y2H screen in a human embryonic library using preys designed to ‘coiled-coil domain4’ and the disordered domain. While baits from the ‘coiled-coil domain4’ screen suggest this region mediates localisation to the centrosome, results from the disordered domain suggest that this region may mediate regulation of Hedgehog signalling.
Original languageEnglish
Pages17
Number of pages1
Publication statusPublished - 1 Feb 2012
Event3rd Annual Nordic Cilia and Centrosome Meeting - Sigtuna, Sweden
Duration: 1 Feb 20133 Mar 2013

Conference

Conference3rd Annual Nordic Cilia and Centrosome Meeting
Country/TerritorySweden
CitySigtuna
Period1/02/133/03/13

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