The genome sequence of the lesser marbled fritillary, Brenthis ino, and evidence for a segregating neo-Z chromosome

Alex MacKintosh; Dominik R Laetsch; Tobias Baril; Robert Foster; Vlad Dinca; Roger Vila; Alexander Hayward; Konrad Lohse

doi:10.1093/g3journal/jkac069

The genome sequence of the lesser marbled fritillary, Brenthis ino, and evidence for a segregating neo-Z chromosome

Alex MacKintosh, Dominik R Laetsch, Tobias Baril, Robert Foster, Vlad Dinca, Roger Vila, Alexander Hayward, Konrad Lohse

Research output: Contribution to journal › Article › peer-review

Abstract

The lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is a species of Palearctic butterfly. Male Brenthis ino individuals have been reported to have between 12 and 14 pairs of chromosomes, a much-reduced chromosome number than is typical in butterflies. Here, we present a chromosome-level genome assembly for Brenthis ino, as well as gene and transposable element annotations. The assembly is 411.8 Mb in length with a contig N50 of 9.6 Mb and a scaffold N50 of 29.5 Mb. We also show evidence that the male individual from which we generated HiC data was heterozygous for a neo-Z chromosome, consistent with inheriting 14 chromosomes from one parent and 13 from the other. This genome assembly will be a valuable resource for studying chromosome evolution in Lepidoptera, as well as for comparative and population genomics more generally.

Original language	English
Article number	jkac069
Number of pages	8
Journal	G3: Genes \| Genomes \| Genetics
Volume	12
Issue number	6
Early online date	28 Mar 2022
DOIs	https://doi.org/10.1093/g3journal/jkac069
Publication status	Published - 28 Mar 2022

Keywords / Materials (for Non-textual outputs)

brenthis ino
genome assembly
genome annotation
neo Z

Access to Document

10.1093/g3journal/jkac069Licence: Creative Commons: Attribution (CC-BY)

LohseEtalG32022TheGenomeSequenceOfTheLesserMarbledFritillaryFinal published version, 1.7 MBLicence: Creative Commons: Attribution (CC-BY)

Modelling the genomic landscapes of selection and speciation
Lohse, K. (Principal Investigator)
EU government bodies
1/02/18 → 31/01/24
Project: Research
Using genomes to dissect the speciation process - a comparative approach
Lohse, K. (Principal Investigator)
NERC
31/12/14 → 30/11/21
Project: Research

Cite this

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title = "The genome sequence of the lesser marbled fritillary, Brenthis ino, and evidence for a segregating neo-Z chromosome",

abstract = "The lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is a species of Palearctic butterfly. Male Brenthis ino individuals have been reported to have between 12 and 14 pairs of chromosomes, a much-reduced chromosome number than is typical in butterflies. Here, we present a chromosome-level genome assembly for Brenthis ino, as well as gene and transposable element annotations. The assembly is 411.8 Mb in length with a contig N50 of 9.6 Mb and a scaffold N50 of 29.5 Mb. We also show evidence that the male individual from which we generated HiC data was heterozygous for a neo-Z chromosome, consistent with inheriting 14 chromosomes from one parent and 13 from the other. This genome assembly will be a valuable resource for studying chromosome evolution in Lepidoptera, as well as for comparative and population genomics more generally.",

keywords = "brenthis ino, genome assembly, genome annotation, neo Z",

author = "Alex MacKintosh and Laetsch, \{Dominik R\} and Tobias Baril and Robert Foster and Vlad Dinca and Roger Vila and Alexander Hayward and Konrad Lohse",

note = "Funding Information: AM is supported by an E4 PhD studentship from the Natural Environment Research Council (NERC, NE/S007407/1). KL is supported by a fellowship from the Natural Environment Research Council (NERC, NE/L011522/1). RV is supported by Grant PID2019-107078GB-I00 funded by Ministerio de Ciencia e Innovaci{\'o}n and Agencia Estatal de Investigaci{\'o}n (MCIN/AEI/10.13039/ 501100011033). VD is supported by the Academy of Finland (Academy Research Fellow, decision no. 328895). This work was supported by a European Research Council starting grant (ModelGenomLand 757648) to KL and a David Phillips Fellowship (BB/N020146/1) by the Biotechnology and Biological Sciences Research Council (BBSRC) to AH. Publisher Copyright: {\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America",

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AU - Foster, Robert

AU - Dinca, Vlad

AU - Vila, Roger

AU - Hayward, Alexander

AU - Lohse, Konrad

N1 - Funding Information: AM is supported by an E4 PhD studentship from the Natural Environment Research Council (NERC, NE/S007407/1). KL is supported by a fellowship from the Natural Environment Research Council (NERC, NE/L011522/1). RV is supported by Grant PID2019-107078GB-I00 funded by Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (MCIN/AEI/10.13039/ 501100011033). VD is supported by the Academy of Finland (Academy Research Fellow, decision no. 328895). This work was supported by a European Research Council starting grant (ModelGenomLand 757648) to KL and a David Phillips Fellowship (BB/N020146/1) by the Biotechnology and Biological Sciences Research Council (BBSRC) to AH. Publisher Copyright: © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America

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The genome sequence of the lesser marbled fritillary, Brenthis ino, and evidence for a segregating neo-Z chromosome

Abstract

Keywords / Materials (for Non-textual outputs)

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Projects

Modelling the genomic landscapes of selection and speciation

Using genomes to dissect the speciation process - a comparative approach

Cite this