Abstract / Description of output
Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We demonstrate that in N. crassa the rcm-1 and rco-1 genes are required for hyphal fusion and normal hyphal morphology, and influence both asexual and sexual development. The ham-5 gene encodes a 1686 amino acid protein with two putative WD40 domains, which might participate in protein-protein interactions. ham-5 deletion mutants had a reduced rate of hyphal extension and altered hyphal morphology, and were unable to produce the conidial anastomosis tubes that are required for hyphal fusion during colony initiation.
Original language | English |
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Pages (from-to) | 2621-2629 |
Number of pages | 9 |
Journal | Microbiology |
Volume | 156 |
Issue number | Pt9 |
Early online date | 3 Jun 2010 |
DOIs | |
Publication status | Published - 1 Sept 2010 |
Keywords / Materials (for Non-textual outputs)
- CAT, conidial anastomosis tube
- DIC, differential interference contrast
- RIP, repeat-induced point mutation