The impact of molecular morphology techniques on the expert diagnosis in melanocytic skin neoplasms

Gerardo Ferrara, Cosimo Misciali, Thomas Brenn, Lorenzo Cerroni, Dmitry W Kazakov, Antonio Perasole, Rosa Russo, Roberto Ricci, Giuliana Crisman, Pier Alessandro Fanti, Beatrice Passarini, Annalisa Patrizi

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Thirteen melanocytic skin neoplasms with a consultation diagnosis by A. Bernard Ackerman were submitted to immunohistochemistry for HMB-45, Ki67, cyclin D1, e-cadherin, and p16; 9/13 cases underwent fluorescence in situ hybridization (FISH) test targeting 6p25 (RREB1), 6q23 (MYB), centromere 6 (Cep6), and 11q13 (CCND1), as well as the centromere 7 (Cep7). A "consensus diagnosis" among 3 experts was also advocated both before and after morphomolecular information. Three neoplasms with a consultation diagnosis of Spitz nevus showed at least 3 abnormal immunohistochemical patterns; 2 of these cases were also FISH-positive for CCND1 gain, but none of them had a final consensus diagnosis of melanoma. Two neoplasms with a consultation diagnosis of congenital nevus received a consensus diagnosis of melanoma. Molecular morphology techniques can highlight the atypical features of melanocytic neoplasms and support existence of a morphobiologic "spectrum": This should be mirrored in the final report by abandoning the dichotomic (benign vs malignant) diagnostic approach.

Original languageEnglish
Pages (from-to)483-92
Number of pages10
JournalInternational Journal of Surgical Pathology
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 2013

Keywords / Materials (for Non-textual outputs)

  • Adult
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Male
  • Melanoma
  • Middle Aged
  • Nevus, Pigmented
  • Skin Neoplasms
  • Tumor Markers, Biological

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