The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens

Chelsea N. Davis, Helen Phillips, John J. Tomes, Martin T. Swain, Toby Wilkinson, Peter Brophy, Russell M Morphew

Research output: Contribution to journalArticlepeer-review

Abstract

Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-
secretory products are necessary for downstream studies and application development.
The most widely used purification method of EVs for helminth pathogens is currently
differential centrifugation (DC). In contrast, size exclusion chromatography (SEC) has been
included in the purification pipeline for EVs from other pathogens, highlighting there is not an
agreed research community ‘gold standard’ for EV isolation. In this case study, Fasciola
hepatica from natural populations were cultured in order to collect EVs from culture media
and evaluate a SEC or DC approach to pathogen helminth EV purification
Original languageEnglish
JournalPLoS Neglected Tropical Diseases
DOIs
Publication statusPublished - 27 Feb 2019

Fingerprint Dive into the research topics of 'The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens'. Together they form a unique fingerprint.

Cite this