The Influence of Lamellar Orientation on Corneal Material Behaviour - Biomechanical and Structural Changes in an Avian Corneal Disorder

C. Boote, A. Elsheikh, W. Kassem, C.S. Kamma-Lorger, P.M. Hocking, N. White, C.F. Inglehearn, M. Ali, K.M. Meek

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: Retinopathy, globe enlarged (RGE) is an inherited genetic disease of chickens with a corneal phenotype characterized by loss of tissue curvature and changes in peripheral collagen fibril alignment. Here we aimed to characterize the material behaviour of normal and RGE chicken corneas under inflation, and compare this with new spatial- and depth-resolved microstructural information, in order to investigate how stromal fibril architecture determines corneal behaviour under intraocular pressure (IOP). Methods: Six RGE chicken corneas and six age-matched normal controls were tested using trephinate inflation and their stress-strain behaviour determined as a function of posterior pressure. Second harmonic generation mulitphoton microscopy was used to compare the in-plane appearance and degree of through-plane interlacing of collagen lamellae between normal and mutant corneas. Results: RGE corneas displayed a 30 - 130% increase in material stiffness (Etangent(RGE) = 0.94 +/- 0.18 MPa - 3.09 +/- 0.66 MPa; Etangent(normals) = 0.72 +/- 0.13 MPa - 1.34 +/- 0.35 MPa) (P
Original languageUndefined/Unknown
Pages (from-to)1243-1251
Number of pages9
JournalInvestigative Ophthalmology & Visual Science
Volume52
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • mechanical anisotropy porcine cornea stroma retinopathy chickens architecture morphogenesis organization maintenance phenotype

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