The Ixodes scapularis salivary protein, salp15, prevents the association of HIV-1 gp120 and CD4

I. J. Juncadella, R. Garg, T. C. Bates, E. R. Olivera, J. Anguita

Research output: Contribution to journalArticlepeer-review

Abstract

Ixodes scapularis salivary protein, Salp15, inhibits CD4(+) T cell activation by binding to the most-extracellular domains of the CD4 molecule, potentially overlapping with the gp120-binding region. We now show that Salp15 inhibits the interaction of gp120 and CD4. Furthermore, Salp15 prevents syncytia formation between HL2/3 (a stable HeLa cell line expressing the envelope protein) and CD4-expressing cells. Salp 15 prevented gp120-CD4 interaction at least partially through its direct interaction with the envelope glycoprotein. A phage display library screen provided the interacting residues in the C1 domain of gp120. These results provide a potential basis to define exposed gp120 epitopes for the generation of neutralizing vaccines. (C) 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number1
DOIs
Publication statusPublished - Feb 2008

Keywords

  • Salp15
  • HIV
  • gp120-CD4 interaction

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