The longevity of biologically-active oligogalacturonides in rose cell cultures: Degradation by exo-polygalacturonase

I GarciaRomera, S C Fry

Research output: Contribution to journalArticlepeer-review

Abstract

To study the metabolism of oligosaccharins, we monitored the fate of (6-C-14)-labelled oligo-alpha-(1-->4)-D-galacturonides of degree of polymerization 8 and 9 (GalA(8) and GalA(9)) when administered at similar to 1 mu M to cell suspension cultures of 'Paul's Scarlet' rose (Rosa sp.). Neither GalA, nor GalA, was appreciably taken up by or bound to the cells. Both GalA(8) and GalA(9) were rapidly hydrolysed by the culture (half-life 2-4 h). The products were smaller oligogalacturonides and galacturonic acid, indicating the action of exo-polygalacturonase. It is suggested that exo-polygalacturonase plays an important role in controlling the concentrations of biologically-active oligogalacturonides in uninfected plant tissues.

Original languageEnglish
Pages (from-to)1853-1857
Number of pages5
JournalJournal of Experimental Botany
Volume46
Issue number293
Publication statusPublished - Dec 1995

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