Projects per year
Abstract
The development and continuous improvement of high-throughput sequencing platforms has stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is
described and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.
described and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.
Original language | English |
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Article number | e02627-17 |
Number of pages | 12 |
Journal | Applied and Environmental Microbiology |
Volume | 84 |
Issue number | 8 |
Early online date | 2 Feb 2018 |
DOIs | |
Publication status | Published - Apr 2018 |
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Dive into the research topics of 'The madness of microbiome: Attempting to find consensus “best practice” for 16S microbiome studies'. Together they form a unique fingerprint.Projects
- 6 Finished
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Understanding the functional and genomic architecture of the rumen microbiome affecting performance traits in bovines
1/12/16 → 30/11/19
Project: Research