The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine

John C. Schwartz; Johanneke Hemmink; Simon P. Graham; Elma Tchilian; Bryan Charleston; Sabine E. Hammer; Chak-Sum Ho; John A. Hammond

doi:10.1111/tan.13281

The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine

John C. Schwartz, Johanneke Hemmink, Simon P. Graham, Elma Tchilian, Bryan Charleston, Sabine E. Hammer, Chak-Sum Ho, John A. Hammond

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

The Babraham pig is a highly inbred breed first developed in the United Kingdom
approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the MHC region, but confirmation of homozygosity at thespecific MHC loci was lacking. Using both direct sequencing and PCR-based sequence specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalize their MHC haplotype as Hp-55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic
35 variation is important.

Original language	English
Pages (from-to)	40-43
Journal	Tissue antigens
Volume	92
Issue number	1
Early online date	23 Apr 2018
DOIs	https://doi.org/10.1111/tan.13281
Publication status	Published - Jul 2018

Keywords / Materials (for Non-textual outputs)

genotyping
SSP typing
Sus scrofa
swine leukocyte antigen
SLA

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10.1111/tan.13281

2018.04.09 HLA submission[2]Accepted author manuscript, 261 KB

https://onlinelibrary.wiley.com/doi/abs/10.1111/tan.13281

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title = "The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine",

abstract = "The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the MHC region, but confirmation of homozygosity at thespecific MHC loci was lacking. Using both direct sequencing and PCR-based sequence specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalize their MHC haplotype as Hp-55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic 35 variation is important.",

keywords = "genotyping, SSP typing, Sus scrofa, swine leukocyte antigen, SLA",

author = "Schwartz, \{John C.\} and Johanneke Hemmink and Graham, \{Simon P.\} and Elma Tchilian and Bryan Charleston and Hammer, \{Sabine E.\} and Chak-Sum Ho and Hammond, \{John A.\}",

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volume = "92",

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T1 - The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine

AU - Schwartz, John C.

AU - Hemmink, Johanneke

AU - Graham, Simon P.

AU - Tchilian, Elma

AU - Charleston, Bryan

AU - Hammer, Sabine E.

AU - Ho, Chak-Sum

AU - Hammond, John A.

PY - 2018/7

Y1 - 2018/7

N2 - The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the MHC region, but confirmation of homozygosity at thespecific MHC loci was lacking. Using both direct sequencing and PCR-based sequence specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalize their MHC haplotype as Hp-55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic 35 variation is important.

AB - The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the MHC region, but confirmation of homozygosity at thespecific MHC loci was lacking. Using both direct sequencing and PCR-based sequence specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalize their MHC haplotype as Hp-55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic 35 variation is important.

KW - genotyping

KW - SSP typing

KW - Sus scrofa

KW - swine leukocyte antigen

KW - SLA

U2 - 10.1111/tan.13281

DO - 10.1111/tan.13281

M3 - Comment/debate

SN - 0001-2815

VL - 92

SP - 40

EP - 43

JO - Tissue antigens

JF - Tissue antigens

IS - 1

ER -

The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine

Abstract

Keywords / Materials (for Non-textual outputs)

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