The MatP/matS Site-Specific System Organizes the Terminus Region of the E. coli Chromosome into a Macrodomain

Romain Mercier, Marie-Agnès Petit, Sophie Schbath, Stéphane Robin, Meriem El Karoui, Frédéric Boccard, Olivier Espéli

Research output: Contribution to journalArticlepeer-review

Abstract

The organization of the Escherichia coli chromosome into insulated macrodomains influences the segregation of sister chromatids and the mobility of chromosomal DNA. Here, we report that organization of the Terminus region (Ter) into a macrodomain relies on the presence of a 13 bp motif called matS repeated 23 times in the 800-kb-long domain. matS sites are the main targets in the E. coli chromosome of a newly identified protein designated MatP. MatP accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP, DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. Our results indicate that a specific organizational system is required in the Terminus region for bacterial chromosome management during the cell cycle.
Original languageEnglish
Pages (from-to)475-485
Number of pages11
JournalCell
Volume135
Issue number3
DOIs
Publication statusPublished - 2008

Keywords

  • CELLCYCLE
  • CELLBIO
  • MICROBIO

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