TY - JOUR
T1 - The microtubule-binding protein Cep170 promotes the targeting of the kinesin-13 depolymerase Kif2b to the mitotic spindle
AU - Welburn, J.P.I.
AU - Cheeseman, I.M.
PY - 2012/12/15
Y1 - 2012/12/15
N2 - Microtubule dynamics are essential throughout mitosis to ensure correct chro mosome segregation. Microtubule depolymerization is controlled in part by microtubule de polymerases, including the kinesin-13 family of proteins. In humans, there are three closely related kinesin-13 isoforms (Kif2a, Kif2b, and Kif2c/MCAK), which are highly conserved in their primary sequences but display distinct localization and nonoverlapping functions. Here we demonstrate that the N-terminus is a primary determinant of kinesin-13 localization. How ever, we also find that differences in the C-terminus alter the properties of kinesin-13, in part by facilitating unique protein-protein interactions. We identify the spindle-localized proteins Cep170 and Cep170R (KIAA0284) as specifically associating with Kif2b. Cep170 binds to microtubules in vitro and provides Kif2b with a second microtubule-binding site to target it to the spindle. Thus the intrinsic properties of kinesin-13s and extrinsic factors such as their associated proteins result in the diversity and specificity within the kinesin-13 depolymerase family.
AB - Microtubule dynamics are essential throughout mitosis to ensure correct chro mosome segregation. Microtubule depolymerization is controlled in part by microtubule de polymerases, including the kinesin-13 family of proteins. In humans, there are three closely related kinesin-13 isoforms (Kif2a, Kif2b, and Kif2c/MCAK), which are highly conserved in their primary sequences but display distinct localization and nonoverlapping functions. Here we demonstrate that the N-terminus is a primary determinant of kinesin-13 localization. How ever, we also find that differences in the C-terminus alter the properties of kinesin-13, in part by facilitating unique protein-protein interactions. We identify the spindle-localized proteins Cep170 and Cep170R (KIAA0284) as specifically associating with Kif2b. Cep170 binds to microtubules in vitro and provides Kif2b with a second microtubule-binding site to target it to the spindle. Thus the intrinsic properties of kinesin-13s and extrinsic factors such as their associated proteins result in the diversity and specificity within the kinesin-13 depolymerase family.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84871207637&partnerID=8YFLogxK
U2 - 10.1091/mbc.E12-03-0214
DO - 10.1091/mbc.E12-03-0214
M3 - Article
AN - SCOPUS:84871207637
SN - 1059-1524
VL - 23
SP - 4786
EP - 4795
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 24
ER -