The miR-144/451(eGFP) allele, a novel tool for resolving the erythroid potential of hematopoietic precursors

Kasper D. Rasmussen, Donal O'Carroll*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A long outstanding problem is the resolution of the full potential of hematopoietic precursors. The commonly used allotypic marker Ly5 permits the tracing of lymphoid and granulocyte-macrophage (GM) output. Here we present a novel eGFP allele that allows the quantitative analysis of red blood cell (RBC) origin at the single-cell level. The miR-144/451 locus is required for erythroid development and homeostasis. Taking advantage of the fact that miR-451 is specifically and highly expressed in the erythroid lineage, we inserted an eGFP expression cassette into the miR144/451 locus. In miR-144/451(+/eGFP) animals, accumulation of eGFP is exclusively observed during terminal erythroid differentiation. Expression of miR-144/451(eGFP) ignites immediately before the CFU-E stage and results in strong and complete labeling of all mature RBCs in circulation. Using competitive reconstitution experiments in the Ly5 transplant model, we show that eGFP linearly correlates with Ly5 expression. Thus, the miR-144/451(eGFP) allele represents a novel tool for the resolution of erythroid potential. 

Original languageEnglish
Pages (from-to)2988-2992
Number of pages5
JournalBlood
Volume118
Issue number11
Early online date25 Jul 2011
DOIs
Publication statusPublished - 15 Sep 2011

Keywords

  • STEM-CELLS
  • LINEAGE COMMITMENT
  • BONE-MARROW
  • MICE
  • PROGENITOR
  • PATHWAY
  • BLOOD
  • DIFFERENTIATION
  • ERYTHROPOIESIS
  • IDENTIFICATION
  • alleles
  • erythrocytes
  • retail clinics
  • transplantation
  • erythroid progenitor cells
  • granulocytes
  • macrophages
  • homeostasis

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