TY - JOUR
T1 - The Nell-1 Growth Factor Stimulates Bone Formation by Purified Human Perivascular Cells
AU - Zhang, Xinli
AU - Peault, Bruno
AU - Chen, Weiwei
AU - Li, Weiming
AU - Corselli, Mirko
AU - James, Aaron W.
AU - Lee, Min
AU - Siu, Ronald K.
AU - Shen, Pang
AU - Zheng, Zhong
AU - Shen, Jia
AU - Kwak, Jinny
AU - Zara, Janette N.
AU - Chen, Feng
AU - Zhang, Hong
AU - Yin, Zack
AU - Wu, Ben
AU - Ting, Kang
AU - Soo, Chia
PY - 2011/10
Y1 - 2011/10
N2 - The search for novel sources of stem cells other than bone marrow mesenchymal stem cells (MSCs) for bone regeneration and repair has been a critical endeavor. We previously established an effective protocol to homogeneously purify human pericytes from multiple fetal and adult tissues, including adipose, bone marrow, skeletal muscle, and pancreas, and identified pericytes as a primitive origin of human MSCs. In the present study, we further characterized the osteogenic potential of purified human pericytes combined with a novel osteoinductive growth factor, Nell-1. Purified pericytes grown on either standard culture ware or human cancellous bone chip (hCBC) scaffolds exhibited robust osteogenic differentiation in vitro. Using a nude mouse muscle pouch model, pericytes formed significant new bone in vivo as compared to scaffold alone (hCBC). Moreover, Nell-1 significantly increased pericyte osteogenic differentiation, both in vitro and in vivo. Interestingly, Nell-1 significantly induced pericyte proliferation and was observed to have pro-angiogenic effects, both in vitro and in vivo. These studies suggest that pericytes are a potential new cell source for future efforts in skeletal regenerative medicine, and that Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation.
AB - The search for novel sources of stem cells other than bone marrow mesenchymal stem cells (MSCs) for bone regeneration and repair has been a critical endeavor. We previously established an effective protocol to homogeneously purify human pericytes from multiple fetal and adult tissues, including adipose, bone marrow, skeletal muscle, and pancreas, and identified pericytes as a primitive origin of human MSCs. In the present study, we further characterized the osteogenic potential of purified human pericytes combined with a novel osteoinductive growth factor, Nell-1. Purified pericytes grown on either standard culture ware or human cancellous bone chip (hCBC) scaffolds exhibited robust osteogenic differentiation in vitro. Using a nude mouse muscle pouch model, pericytes formed significant new bone in vivo as compared to scaffold alone (hCBC). Moreover, Nell-1 significantly increased pericyte osteogenic differentiation, both in vitro and in vivo. Interestingly, Nell-1 significantly induced pericyte proliferation and was observed to have pro-angiogenic effects, both in vitro and in vivo. These studies suggest that pericytes are a potential new cell source for future efforts in skeletal regenerative medicine, and that Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation.
U2 - 10.1089/ten.tea.2010.0705
DO - 10.1089/ten.tea.2010.0705
M3 - Article
SN - 1937-3341
VL - 17
SP - 2497
EP - 2509
JO - Tissue Engineering Part A
JF - Tissue Engineering Part A
IS - 19-20
ER -