The NEMP family supports metazoan fertility and nuclear envelope stiffness

Yonit Tsatskis; Robyn Rosenfeld; Joel D. Pearson; Curtis Boswell; Yi Qu; Kyunga Kim; Lacramioara Fabian; Ariz Mohammed; Xian Wang; Michael Robson; Karen Krchma; Jun Wu; João Gonçalves; Didier Hodzic; Shu Wu; Daniel Potter; Laurence Pelletier; Wade H. Dunham; Anne-Claude Gingras; Yu Sun; Jin Meng; Dorothea Godt; Tim Schedl; Brian Ciruna; Kyunghee Choi; John R B Perry; Rod Bremner; Eric C. Schirmer; Julie A. Brill; Andrea Jurisicova; Helen McNeill

doi:10.1126/sciadv.abb4591

The NEMP family supports metazoan fertility and nuclear envelope stiffness

Yonit Tsatskis, Robyn Rosenfeld, Joel D. Pearson, Curtis Boswell, Yi Qu, Kyunga Kim, Lacramioara Fabian, Ariz Mohammed, Xian Wang, Michael Robson, Karen Krchma, Jun Wu, João Gonçalves, Didier Hodzic, Shu Wu, Daniel Potter, Laurence Pelletier, Wade H. Dunham, Anne-Claude Gingras, Yu SunJin Meng, Dorothea Godt, Tim Schedl, Brian Ciruna, Kyunghee Choi, John R B Perry, Rod Bremner, Eric C. Schirmer, Julie A. Brill, Andrea Jurisicova, Helen McNeill

Research output: Contribution to journal › Article › peer-review

Abstract

Human Genome Wide Association Studies have linked SNPs in NEMP1 (Nuclear Envelope Membrane Protein 1) with early menopause (1,2), however it is unclear if NEMP1 has any role in fertility. We show that whole animal loss of NEMP1 homologs in Drosophila, C. elegans, zebrafish and mice lead to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germline. Biochemical, biophysical and genetic studies reveal NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP
EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties, and that NEMP proteins play essential and conserved roles in fertility.

Original language	English
Article number	eabb4591
Journal	Science Advances
Volume	6
DOIs	https://doi.org/10.1126/sciadv.abb4591
Publication status	Published - 28 Aug 2020

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McNeillEtalSA2020TheNempFamilySupportsMetazoanFertilityAndNuclearEnvelopeFinal published version, 3.46 MBLicence: Creative Commons: Attribution Non-Commercial (CC-BY-NC)

2 Finished

Core funding renewal for the Wellcome Trust Centre for Cell Biology
Tollervey, D. & Earnshaw, B.
UK-based charities
1/10/11 → 30/04/17
Project: Research
Nuclear envelope transmembrane protein regulation of tissue-specific genome organisation and cell cycle regulation
Schirmer, E.
UK-based charities
1/08/11 → 31/07/19
Project: Research

Cite this

Tsatskis, Y., Rosenfeld, R., Pearson, J. D., Boswell, C., Qu, Y., Kim, K., Fabian, L., Mohammed, A., Wang, X., Robson, M., Krchma, K., Wu, J., Gonçalves, J., Hodzic, D., Wu, S., Potter, D., Pelletier, L., Dunham, W. H., Gingras, A-C., ... McNeill, H. (2020). The NEMP family supports metazoan fertility and nuclear envelope stiffness. Science Advances, 6, [eabb4591]. https://doi.org/10.1126/sciadv.abb4591

Tsatskis, Yonit ; Rosenfeld, Robyn ; Pearson, Joel D. ; Boswell, Curtis ; Qu, Yi ; Kim, Kyunga ; Fabian, Lacramioara ; Mohammed, Ariz ; Wang, Xian ; Robson, Michael ; Krchma, Karen ; Wu, Jun ; Gonçalves, João ; Hodzic, Didier ; Wu, Shu ; Potter, Daniel ; Pelletier, Laurence ; Dunham, Wade H. ; Gingras, Anne-Claude ; Sun, Yu ; Meng, Jin ; Godt, Dorothea ; Schedl, Tim ; Ciruna, Brian ; Choi, Kyunghee ; Perry, John R B ; Bremner, Rod ; Schirmer, Eric C. ; Brill, Julie A. ; Jurisicova, Andrea ; McNeill, Helen. / The NEMP family supports metazoan fertility and nuclear envelope stiffness. In: Science Advances. 2020 ; Vol. 6.

@article{dbad545af8a94a6f8aef0c0da0f5ff18,

title = "The NEMP family supports metazoan fertility and nuclear envelope stiffness",

abstract = "Human Genome Wide Association Studies have linked SNPs in NEMP1 (Nuclear Envelope Membrane Protein 1) with early menopause (1,2), however it is unclear if NEMP1 has any role in fertility. We show that whole animal loss of NEMP1 homologs in Drosophila, C. elegans, zebrafish and mice lead to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germline. Biochemical, biophysical and genetic studies reveal NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties, and that NEMP proteins play essential and conserved roles in fertility.",

author = "Yonit Tsatskis and Robyn Rosenfeld and Pearson, {Joel D.} and Curtis Boswell and Yi Qu and Kyunga Kim and Lacramioara Fabian and Ariz Mohammed and Xian Wang and Michael Robson and Karen Krchma and Jun Wu and Jo{\~a}o Gon{\c c}alves and Didier Hodzic and Shu Wu and Daniel Potter and Laurence Pelletier and Dunham, {Wade H.} and Anne-Claude Gingras and Yu Sun and Jin Meng and Dorothea Godt and Tim Schedl and Brian Ciruna and Kyunghee Choi and Perry, {John R B} and Rod Bremner and Schirmer, {Eric C.} and Brill, {Julie A.} and Andrea Jurisicova and Helen McNeill",

year = "2020",

month = aug,

day = "28",

doi = "10.1126/sciadv.abb4591",

language = "English",

volume = "6",

journal = "Science Advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

}

Tsatskis, Y, Rosenfeld, R, Pearson, JD, Boswell, C, Qu, Y, Kim, K, Fabian, L, Mohammed, A, Wang, X, Robson, M, Krchma, K, Wu, J, Gonçalves, J, Hodzic, D, Wu, S, Potter, D, Pelletier, L, Dunham, WH, Gingras, A-C, Sun, Y, Meng, J, Godt, D, Schedl, T, Ciruna, B, Choi, K, Perry, JRB, Bremner, R, Schirmer, EC, Brill, JA, Jurisicova, A & McNeill, H 2020, 'The NEMP family supports metazoan fertility and nuclear envelope stiffness', Science Advances, vol. 6, eabb4591. https://doi.org/10.1126/sciadv.abb4591

The NEMP family supports metazoan fertility and nuclear envelope stiffness. / Tsatskis, Yonit; Rosenfeld, Robyn; Pearson, Joel D.; Boswell, Curtis; Qu, Yi; Kim, Kyunga; Fabian, Lacramioara; Mohammed, Ariz; Wang, Xian; Robson, Michael; Krchma, Karen; Wu, Jun; Gonçalves, João; Hodzic, Didier; Wu, Shu; Potter, Daniel; Pelletier, Laurence; Dunham, Wade H.; Gingras, Anne-Claude; Sun, Yu; Meng, Jin; Godt, Dorothea; Schedl, Tim; Ciruna, Brian; Choi, Kyunghee; Perry, John R B; Bremner, Rod; Schirmer, Eric C.; Brill, Julie A.; Jurisicova, Andrea; McNeill, Helen.

In: Science Advances, Vol. 6, eabb4591, 28.08.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The NEMP family supports metazoan fertility and nuclear envelope stiffness

AU - Tsatskis, Yonit

AU - Rosenfeld, Robyn

AU - Pearson, Joel D.

AU - Boswell, Curtis

AU - Qu, Yi

AU - Kim, Kyunga

AU - Fabian, Lacramioara

AU - Mohammed, Ariz

AU - Wang, Xian

AU - Robson, Michael

AU - Krchma, Karen

AU - Wu, Jun

AU - Gonçalves, João

AU - Hodzic, Didier

AU - Wu, Shu

AU - Potter, Daniel

AU - Pelletier, Laurence

AU - Dunham, Wade H.

AU - Gingras, Anne-Claude

AU - Sun, Yu

AU - Meng, Jin

AU - Godt, Dorothea

AU - Schedl, Tim

AU - Ciruna, Brian

AU - Choi, Kyunghee

AU - Perry, John R B

AU - Bremner, Rod

AU - Schirmer, Eric C.

AU - Brill, Julie A.

AU - Jurisicova, Andrea

AU - McNeill, Helen

PY - 2020/8/28

Y1 - 2020/8/28

N2 - Human Genome Wide Association Studies have linked SNPs in NEMP1 (Nuclear Envelope Membrane Protein 1) with early menopause (1,2), however it is unclear if NEMP1 has any role in fertility. We show that whole animal loss of NEMP1 homologs in Drosophila, C. elegans, zebrafish and mice lead to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germline. Biochemical, biophysical and genetic studies reveal NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties, and that NEMP proteins play essential and conserved roles in fertility.

AB - Human Genome Wide Association Studies have linked SNPs in NEMP1 (Nuclear Envelope Membrane Protein 1) with early menopause (1,2), however it is unclear if NEMP1 has any role in fertility. We show that whole animal loss of NEMP1 homologs in Drosophila, C. elegans, zebrafish and mice lead to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germline. Biochemical, biophysical and genetic studies reveal NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties, and that NEMP proteins play essential and conserved roles in fertility.

U2 - 10.1126/sciadv.abb4591

DO - 10.1126/sciadv.abb4591

M3 - Article

VL - 6

JO - Science Advances

JF - Science Advances

SN - 2375-2548

M1 - eabb4591

ER -

The NEMP family supports metazoan fertility and nuclear envelope stiffness

Abstract

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Projects

Core funding renewal for the Wellcome Trust Centre for Cell Biology

Nuclear envelope transmembrane protein regulation of tissue-specific genome organisation and cell cycle regulation

Cite this