The nuclear autoimmune antigen Ku is also present on the cell surface

R G Dalziel; S C Mendelson; J P Quinn

The nuclear autoimmune antigen Ku is also present on the cell surface

R G Dalziel, S C Mendelson, J P Quinn

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.

Original language	English
Pages (from-to)	265-7
Number of pages	3
Journal	Autoimmunity
Volume	13
Issue number	4
Publication status	Published - 1992

Keywords / Materials (for Non-textual outputs)

Antigens, Nuclear
Autoantigens
Cell Membrane
Cell Nucleus
DNA Helicases
DNA-Binding Proteins
Flow Cytometry
HeLa Cells
Humans
Nuclear Proteins
T-Lymphocytes

Cite this

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title = "The nuclear autoimmune antigen Ku is also present on the cell surface",

abstract = "Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.",

keywords = "Antigens, Nuclear, Autoantigens, Cell Membrane, Cell Nucleus, DNA Helicases, DNA-Binding Proteins, Flow Cytometry, HeLa Cells, Humans, Nuclear Proteins, T-Lymphocytes",

author = "Dalziel, {R G} and Mendelson, {S C} and Quinn, {J P}",

year = "1992",

language = "English",

volume = "13",

pages = "265--7",

journal = "Autoimmunity",

issn = "0891-6934",

publisher = "Informa Healthcare",

number = "4",

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TY - JOUR

T1 - The nuclear autoimmune antigen Ku is also present on the cell surface

AU - Dalziel, R G

AU - Mendelson, S C

AU - Quinn, J P

PY - 1992

Y1 - 1992

N2 - Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.

AB - Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.

KW - Antigens, Nuclear

KW - Autoantigens

KW - Cell Membrane

KW - Cell Nucleus

KW - DNA Helicases

KW - DNA-Binding Proteins

KW - Flow Cytometry

KW - HeLa Cells

KW - Humans

KW - Nuclear Proteins

KW - T-Lymphocytes

M3 - Article

C2 - 1472635

SN - 0891-6934

VL - 13

SP - 265

EP - 267

JO - Autoimmunity

JF - Autoimmunity

IS - 4

ER -

The nuclear autoimmune antigen Ku is also present on the cell surface

Abstract

Keywords / Materials (for Non-textual outputs)

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