The oocyte spindle is preserved by 1,2-propanediol during slow freezing

Ching-Chien Chang, Li-Ying Sung, Chih-Jen Lin, Hilton I. Kort, Xiangzhong Yang, X. Cindy Tian, Zsolt Peter Nagy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Objective: To investigate the specific changes in oocyte spindle subjected to severe challenges of low temperature, as well as to examine the effect of cryoprotectants in preserving oocyte spindle during cryopreservation.

Design: In vitro experimental study.

Setting: Academic research laboratory.

Animal(s): B6D2F1 (C57BL/6 X DBA/2) mice.

Intervention(s): Mouse oocytes were cryopreserved using a slow freezing method in a sodium-depleted medium with 1.5 mol/l 1,2-propanediol (PROH) and 0.3 M sucrose. To examine the spindle, oocytes were fixed before, during, and after cryopreservation, and oocytes were analyzed by immunocytochemistry and confocal microscopy.

Result(s): The MII spindle was preserved during the slow freezing, because the cryoprotectant PROH was found to support the organization of MII spindle in resisting the subzero temperature. In contrast, the MII spindle was disassembled gradually during the thawing process with or without PROH. Most of the oocytes were able to recover the MII spindle after thawing, but a portion of thawed oocytes could not sustain the meiotic spindle because of parthenogenetic activation.

Conclusion(S): 1,2-Propanediol can support the organization of MII spindle to defy the subphysiologic temperature; however, the PROH cannot sustain oocyte spindle structure after the subsequent thawing process. (Fertil Steril (R) 2010;93:1430-9. (C)2010 by American Society for Reproductive Medicine.)

Original languageEnglish
Pages (from-to)1430-1439
Number of pages10
JournalFertility and Sterility
Volume93
Issue number5
DOIs
Publication statusPublished - 15 Mar 2010

Keywords / Materials (for Non-textual outputs)

  • Oocyte cryopreservation
  • meiotic spindle
  • 1, 2-propanediol
  • UNFERTILIZED MOUSE OOCYTES
  • 2ND MEIOTIC SPINDLE
  • CHROMOSOMAL-ABNORMALITIES
  • GERMINAL VESICLE
  • CRYOPRESERVATION
  • INVITRO
  • EMBRYOS
  • SODIUM
  • FROZEN
  • FERTILIZATION

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