TY - JOUR
T1 - The oral microbiome and breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in the Ghana Breast Health Study
AU - Wu, Zeni
AU - Byrd, Doratha A
AU - Wan, Yunhu
AU - Ansong, Daniel
AU - Clegg-Lamptey, Joe-Nat
AU - Wiafe-Addai, Beatrice
AU - Edusei, Lawrence
AU - Adjei, Ernest
AU - Titiloye, Nicholas
AU - Dedey, Florence
AU - Aitpillah, Francis
AU - Oppong, Joseph
AU - Vanderpuye, Verna
AU - Osei-Bonsu, Ernest
AU - Dagnall, Casey L
AU - Jones, Kristine
AU - Hutchinson, Amy
AU - Hicks, Belynda D
AU - Ahearn, Thomas U
AU - Shi, Jianxin
AU - Knight, Rob
AU - Biritwum, Richard
AU - Yarney, Joel
AU - Wiafe, Seth
AU - Awuah, Baffour
AU - Nyarko, Kofi
AU - Figueroa, Jonine D
AU - Sinha, Rashmi
AU - Garcia-Closas, Montserrat
AU - Brinton, Louise A
AU - Vogtmann, Emily
N1 - Funding Information:
This work was supported by the Intramural Research Program in the Division of Cancer Epidemiology and Genetics, the US National Institutes of Health (NIH), National Cancer Institute (NCI). The success of this investigation would not have been possible without exceptional teamwork and the diligence of the field staff who oversaw the recruitment, interviews and collection of data from study subjects. Special thanks are due to the following individuals: Korle Bu Teaching Hospital, Accra—Dr. Adu‐Aryee, Obed Ekpedzor, Angela Kenu, Victoria Okyne, Naomi Oyoe Ohene Oti, Evelyn Tay; Komfo Anoyke Teaching Hospital, Kumasi—Marion Alcpaloo, Bernard Arhin, Emmanuel Asiamah, Isaac Boakye, Samuel Ka‐chungu and; Peace and Love Hospital, Kumasi—Samuel Amanama, Emma Abaidoo, Prince Agyapong, Thomas Agyei‐Ansong, Debora Boateng, Margaret Frempong, Bridget Nortey Mensah, Richard Opoku and Kofi Owusu Gyimah. The study was further enhanced by surgical expertise provided by Dr. Lisa Newman of the University of Michigan and by pathological expertise provided by Drs. Stephen Hewitt and Petra Lenz of the National Cancer Institute Dr. Maire A. Duggan from the Cumming School of Medicine, University of Calgary, Canada. Study management assistance was received from Ricardo Diaz, Shelley Niwa, Usha Singh, Ann Truelove and Michelle Brotzman at Westat, Inc. We thank Maura Kate Costello of the Division of Cancer Epidemiology and Genetics, National Cancer Institute for helping to create the graphical abstract. Appreciation is also expressed to the many women who agreed to participate in the study and to provide information and biospecimens in hopes of preventing and improving outcomes of breast cancer in Ghana. This work utilized the computational resources of the NIH HPC Biowulf cluster. ( http://hpc.nih.gov ).
Publisher Copyright:
© 2022 UICC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
PY - 2022/10/15
Y1 - 2022/10/15
N2 - The oral microbiome, like the fecal microbiome, may be related to breast cancer risk. Therefore, we investigated whether the oral microbiome was associated with breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in a case-control study in Ghana. A total of 881 women were included (369 breast cancers, 93 nonmalignant cases and 419 population-based controls). The V4 region of the 16S rRNA gene was sequenced from oral and fecal samples. Alpha-diversity (observed amplicon sequence variants [ASVs], Shannon index and Faith's Phylogenetic Diversity) and beta-diversity (Bray-Curtis, Jaccard and weighted and unweighted UniFrac) metrics were computed. MiRKAT and logistic regression models were used to investigate the case-control associations. Oral sample alpha-diversity was inversely associated with breast cancer and nonmalignant breast disease with odds ratios (95% CIs) per every 10 observed ASVs of 0.86 (0.83-0.89) and 0.79 (0.73-0.85), respectively, compared to controls. Beta-diversity was also associated with breast cancer and nonmalignant breast disease compared to controls (P ≤ .001). The relative abundances of Porphyromonas and Fusobacterium were lower for breast cancer cases compared to controls. Alpha-diversity and presence/relative abundance of specific genera from the oral and fecal microbiome were strongly correlated among breast cancer cases, but weakly correlated among controls. Particularly, the relative abundance of oral Porphyromonas was strongly, inversely correlated with fecal Bacteroides among breast cancer cases (r = -.37, P ≤ .001). Many oral microbial metrics were strongly associated with breast cancer and nonmalignant breast disease, and strongly correlated with fecal microbiome among breast cancer cases, but not controls.
AB - The oral microbiome, like the fecal microbiome, may be related to breast cancer risk. Therefore, we investigated whether the oral microbiome was associated with breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in a case-control study in Ghana. A total of 881 women were included (369 breast cancers, 93 nonmalignant cases and 419 population-based controls). The V4 region of the 16S rRNA gene was sequenced from oral and fecal samples. Alpha-diversity (observed amplicon sequence variants [ASVs], Shannon index and Faith's Phylogenetic Diversity) and beta-diversity (Bray-Curtis, Jaccard and weighted and unweighted UniFrac) metrics were computed. MiRKAT and logistic regression models were used to investigate the case-control associations. Oral sample alpha-diversity was inversely associated with breast cancer and nonmalignant breast disease with odds ratios (95% CIs) per every 10 observed ASVs of 0.86 (0.83-0.89) and 0.79 (0.73-0.85), respectively, compared to controls. Beta-diversity was also associated with breast cancer and nonmalignant breast disease compared to controls (P ≤ .001). The relative abundances of Porphyromonas and Fusobacterium were lower for breast cancer cases compared to controls. Alpha-diversity and presence/relative abundance of specific genera from the oral and fecal microbiome were strongly correlated among breast cancer cases, but weakly correlated among controls. Particularly, the relative abundance of oral Porphyromonas was strongly, inversely correlated with fecal Bacteroides among breast cancer cases (r = -.37, P ≤ .001). Many oral microbial metrics were strongly associated with breast cancer and nonmalignant breast disease, and strongly correlated with fecal microbiome among breast cancer cases, but not controls.
KW - Ghana
KW - breast cancer
KW - fecal microbiome
KW - nonmalignant breast diseases
KW - oral microbiome
U2 - 10.1002/ijc.34145
DO - 10.1002/ijc.34145
M3 - Article
C2 - 35657343
SN - 0020-7136
VL - 151
SP - 1248
EP - 1260
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 8
ER -