Projects per year
Abstract / Description of output
Neutrophils and T cells exist in close proximity in lymph nodes and inflamed tissues during health and disease. They are able to form stable interactions, with profound effects on the phenotype and function of the T cells. However, the outcome of these effects are frequently contradictory; in some systems neutrophils suppress T cell proliferation, in others they are activatory or present antigen directly. Published protocols modelling these interactions in vitro do not reflect the full range of interactions found in vivo; they do not examine how activated and naïve T cells differentially respond to neutrophils, or whether de-granulating or resting neutrophils induce different outcomes. Here, we established a culture protocol to ask these questions with human T cells and autologous neutrophils. We find that resting neutrophils suppress T cell proliferation, activation and cytokine production but that de-granulating neutrophils do not, and neutrophil-released intracellular contents enhance proliferation. Strikingly, we also demonstrate that T cells early in the activation process are susceptible to suppression by neutrophils, while later-stage T cells are not, and naïve T cells do not respond at all. Our protocol therefore allows nuanced analysis of the outcome of interaction of these cells and may explain the contradictory results observed previously.
Original language | English |
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Journal | Frontiers in Immunology |
Volume | 12 |
DOIs | |
Publication status | Published - 30 Mar 2021 |
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Dive into the research topics of 'The Outcome of Neutrophil-T Cell Contact Differs Depending on Activation Status of Both Cell Types'. Together they form a unique fingerprint.Projects
- 3 Finished
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Determination of T cell signalling pathway alterations induced by the neutrophil peptide LL-37
Gwyer Findlay, E.
1/10/17 → 30/09/18
Project: Research
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Cathelicidin is Critical for Pathogenic T cell Development in Multiple Sclerosis
Gwyer Findlay, E.
1/01/17 → 6/08/22
Project: Research
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The role of cyclin-dependent kinase-9 inhibition in promoting the resolution of chronic inflammation
1/05/13 → 30/10/19
Project: Research
Equipment
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Institute for Regeneration and Repair Flow Cytometry Facility
Shonna Johnston (Manager), Fiona Rossi (Manager), Claire Cryer (Other) & Ailsa Laird (Other)
Institute of Regeneration and RepairFacility/equipment: Facility