The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes

T Schafer, D Strauss, E Petfalski, D Tollervey, E Hurt

Research output: Contribution to journalArticlepeer-review

Abstract

Recent reports have increased our knowledge of the consecutive steps during 60S ribosome biogenesis substantially, but 40S subunit formation is less well understood. Here, we investigate the maturation of nucleolar 90S pre-ribosomes into cytoplasmic 40S pre-ribosomes. During the transition from 90S to 40S particles, the majority of non-ribosomal proteins (similar to30 species) dissociate, and significantly fewer factors associate with 40S pre-ribosomes. Notably, some of these components are part of both early 90S and intermediate 40S pre-particles in the nucleolus (e.g. Enp1p, Dim1p and Rrp12p), whereas others (e.g. Rio2p and Nob1p) are found mainly on late cytoplasmic pre-40S subunits. Finally, temperature-sensitive mutants mapping either in earlier (enp1-1) or later (rio2-1) components exhibit defects in the formation and nuclear export of pre-40S subunits. Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis.

Original languageEnglish
Pages (from-to)1370-1380
Number of pages11
JournalEMBO Journal
Volume22
Issue number6
DOIs
Publication statusPublished - 17 Mar 2003

Keywords

  • Enp1
  • nuclear export
  • ribosome biogenesis
  • ribosome export
  • 40S subunit

Cite this