The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex

Marie E Bechler; Anne M Doody; Esther Racoosin; Lin Lin; Kelvin H Lee; William J Brown

doi:10.1083/jcb.200908105

The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex

Marie E Bechler, Anne M Doody, Esther Racoosin, Lin Lin, Kelvin H Lee, William J Brown

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

We report that platelet-activating factor acetylhydrolase (PAFAH) Ib, comprised of two phospholipase A(2) (PLA(2)) subunits, alpha1 and alpha2, and a third subunit, the dynein regulator lissencephaly 1 (LIS1), mediates the structure and function of the Golgi complex. Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution system. Overexpression of wild-type or mutant alpha1 or alpha2 revealed that both PLA(2) activity and LIS1 are important for maintaining Golgi structure. Knockdown of PAFAH Ib subunits fragments the Golgi complex, inhibits tubule-mediated reassembly of intact Golgi ribbons, and slows secretion of cargo. Our results demonstrate a cooperative interplay between the PLA(2) activity of alpha1 and alpha2 with LIS1 to facilitate the functional organization of the Golgi complex, thereby suggesting a model that links phospholipid remodeling and membrane tubulation to dynein-dependent transport.

Original language	English
Pages (from-to)	45-53
Number of pages	9
Journal	Journal of Cell Biology
Volume	190
Issue number	1
DOIs	https://doi.org/10.1083/jcb.200908105
Publication status	Published - 12 Jul 2010

Keywords / Materials (for Non-textual outputs)

1-Alkyl-2-acetylglycerophosphocholine Esterase
Animals
Cattle
Gene Knockdown Techniques
Golgi Apparatus
HeLa Cells
Humans
Male
Microtubule-Associated Proteins
Models, Biological
Multienzyme Complexes
Nerve Tissue Proteins
Rats
Rats, Sprague-Dawley

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10.1083/jcb.200908105

The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex
Copyright © 2010 Bechler et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Final published version, 2.91 MBLicence: Creative Commons: Attribution-NonCommercial-ShareAlike (CC BY-NC-SA)

http://jcb.rupress.org/content/190/1/45

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title = "The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex",

abstract = "We report that platelet-activating factor acetylhydrolase (PAFAH) Ib, comprised of two phospholipase A(2) (PLA(2)) subunits, alpha1 and alpha2, and a third subunit, the dynein regulator lissencephaly 1 (LIS1), mediates the structure and function of the Golgi complex. Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution system. Overexpression of wild-type or mutant alpha1 or alpha2 revealed that both PLA(2) activity and LIS1 are important for maintaining Golgi structure. Knockdown of PAFAH Ib subunits fragments the Golgi complex, inhibits tubule-mediated reassembly of intact Golgi ribbons, and slows secretion of cargo. Our results demonstrate a cooperative interplay between the PLA(2) activity of alpha1 and alpha2 with LIS1 to facilitate the functional organization of the Golgi complex, thereby suggesting a model that links phospholipid remodeling and membrane tubulation to dynein-dependent transport.",

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author = "Bechler, {Marie E} and Doody, {Anne M} and Esther Racoosin and Lin Lin and Lee, {Kelvin H} and Brown, {William J}",

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AU - Bechler, Marie E

AU - Doody, Anne M

AU - Racoosin, Esther

AU - Lin, Lin

AU - Lee, Kelvin H

AU - Brown, William J

PY - 2010/7/12

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N2 - We report that platelet-activating factor acetylhydrolase (PAFAH) Ib, comprised of two phospholipase A(2) (PLA(2)) subunits, alpha1 and alpha2, and a third subunit, the dynein regulator lissencephaly 1 (LIS1), mediates the structure and function of the Golgi complex. Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution system. Overexpression of wild-type or mutant alpha1 or alpha2 revealed that both PLA(2) activity and LIS1 are important for maintaining Golgi structure. Knockdown of PAFAH Ib subunits fragments the Golgi complex, inhibits tubule-mediated reassembly of intact Golgi ribbons, and slows secretion of cargo. Our results demonstrate a cooperative interplay between the PLA(2) activity of alpha1 and alpha2 with LIS1 to facilitate the functional organization of the Golgi complex, thereby suggesting a model that links phospholipid remodeling and membrane tubulation to dynein-dependent transport.

AB - We report that platelet-activating factor acetylhydrolase (PAFAH) Ib, comprised of two phospholipase A(2) (PLA(2)) subunits, alpha1 and alpha2, and a third subunit, the dynein regulator lissencephaly 1 (LIS1), mediates the structure and function of the Golgi complex. Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution system. Overexpression of wild-type or mutant alpha1 or alpha2 revealed that both PLA(2) activity and LIS1 are important for maintaining Golgi structure. Knockdown of PAFAH Ib subunits fragments the Golgi complex, inhibits tubule-mediated reassembly of intact Golgi ribbons, and slows secretion of cargo. Our results demonstrate a cooperative interplay between the PLA(2) activity of alpha1 and alpha2 with LIS1 to facilitate the functional organization of the Golgi complex, thereby suggesting a model that links phospholipid remodeling and membrane tubulation to dynein-dependent transport.

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KW - Gene Knockdown Techniques

KW - Golgi Apparatus

KW - HeLa Cells

KW - Humans

KW - Male

KW - Microtubule-Associated Proteins

KW - Models, Biological

KW - Multienzyme Complexes

KW - Nerve Tissue Proteins

KW - Rats

KW - Rats, Sprague-Dawley

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DO - 10.1083/jcb.200908105

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C2 - 20624900

SN - 0021-9525

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JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 1

ER -

The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex

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