The Processive Kinetics of Gene Conversion in Bacteria: Processivity of Gene Conversion

Paulsson Johan, Meriem El Karoui, Monica Lindell, Diarmaid Hughes

Research output: Contribution to journalArticlepeer-review

Abstract

Gene conversion, non-reciprocal transfer from one homologous sequence to another, is a major force in evolutionary dynamics, promoting co-evolution in gene families, and maintaining similarities between repeated genes. However, the properties of the transfer – where it initiates, how far it proceeds, and how the resulting conversion tracts are affected by mismatch repair – are not well understood. Here we use the duplicate tuf genes in Salmonella as a quantitatively tractable model system for gene conversion. We selected for conversion in multiple different positions of tuf, and examined the resulting distributions of conversion tracts in mismatch repair-deficient and mismatch repair-proficient strains. A simple stochastic model accounting for the essential steps of conversion showed excellent agreement with the data for all selection points using the same value of the conversion processivity, which is the only kinetic parameter of the model. The analysis suggests that gene conversion effectively initiates uniformly at any position within a tuf gene, and proceeds with an effectively uniform conversion processivity in either direction limited by the bounds of the gene.
Original languageEnglish
JournalMolecular Microbiology
Early online date13 Mar 2017
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Tuf genes
  • Salmonella
  • MutS
  • Homologous recombination
  • Co-evolution

Fingerprint

Dive into the research topics of 'The Processive Kinetics of Gene Conversion in Bacteria: Processivity of Gene Conversion'. Together they form a unique fingerprint.

Cite this