The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly

Weronika Borek, Nadine Vincenten, Eris Duro, Vaso Makrantoni, Christos Spanos, Krishna K. Sarangapani, Flavia de Lima Alves, David Kelly, Charles L. Asbury, Juri Rappsilber, Adele L Marston

Research output: Contribution to journalArticlepeer-review

Abstract

Kinetochores direct chromosome segregation in mitosis and meiosis. Faithful gamete formation through meiosis requires that kinetochores take on new functions that impact homolog pairing, recombination and the orientation of kinetochore attachment to microtubules in meiosis I. Using an unbiased proteomics pipeline, we determined the composition of centromeric chromatin and kinetochores at distinct cell-cycle stages, revealing extensive reorganisation of kinetochores during meiosis. The data uncover a network of meiotic chromosome axis and recombination proteins that bind to centromeres in the absence of the microtubule-binding outer kinetochore sub-complexes during meiotic prophase. We show that the Ctf19cCCAN inner kinetochore complex is essential for kinetochore organisation in meiosis. Our functional analyses identify a Ctf19cCCAN-dependent kinetochore assembly pathway that is dispensable for mitotic growth but becomes critical upon meiotic entry. Therefore, changes in kinetochore composition and a distinct assembly pathway specialize meiotic kinetochores for successful gametogenesis.
Original languageEnglish
Pages (from-to)P283-296.E7
Number of pages14
JournalCurrent Biology
Volume31
Issue number2
Early online date5 Nov 2020
DOIs
Publication statusPublished - 25 Jan 2021

Keywords

  • meiosis
  • kinetochore
  • centromere
  • proteomics
  • Ctf19
  • CCAN
  • prophase I
  • metaphase I
  • budding yeast

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