Projects per year
Abstract
Kinetochores direct chromosome segregation in mitosis and meiosis. Faithful gamete formation through meiosis requires that kinetochores take on new functions that impact homolog pairing, recombination and the orientation of kinetochore attachment to microtubules in meiosis I. Using an unbiased proteomics pipeline, we determined the composition of centromeric chromatin and kinetochores at distinct cell-cycle stages, revealing extensive reorganisation of kinetochores during meiosis. The data uncover a network of meiotic chromosome axis and recombination proteins that bind to centromeres in the absence of the microtubule-binding outer kinetochore sub-complexes during meiotic prophase. We show that the Ctf19cCCAN inner kinetochore complex is essential for kinetochore organisation in meiosis. Our functional analyses identify a Ctf19cCCAN-dependent kinetochore assembly pathway that is dispensable for mitotic growth but becomes critical upon meiotic entry. Therefore, changes in kinetochore composition and a distinct assembly pathway specialize meiotic kinetochores for successful gametogenesis.
Original language | English |
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Pages (from-to) | P283-296.E7 |
Number of pages | 14 |
Journal | Current Biology |
Volume | 31 |
Issue number | 2 |
Early online date | 5 Nov 2020 |
DOIs | |
Publication status | Published - 25 Jan 2021 |
Keywords / Materials (for Non-textual outputs)
- meiosis
- kinetochore
- centromere
- proteomics
- Ctf19
- CCAN
- prophase I
- metaphase I
- budding yeast
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Dive into the research topics of 'The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly'. Together they form a unique fingerprint.Projects
- 7 Finished
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Wellcome Centre for Cell Biology
Tollervey, D. (Principal Investigator)
1/12/16 → 1/12/21
Project: Research
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Mechanisms orienting chromosomes in mitosis and meiosis
Marston, A. (Principal Investigator)
1/03/16 → 28/08/21
Project: Research
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Proteomics at the Wellcome Trust Centre for Cell Biology (WTCCB) and School of Biological Sciences (SBS), Edinburgh
Rappsilber, J. (Principal Investigator)
1/10/15 → 30/09/20
Project: Research
Profiles
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Adele Marston
- School of Biological Sciences - Personal Chair of Cell Biology
Person: Academic: Research Active