The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

S B Buonomo; A Michienzi; Gian Fabrizio De Angelis; I  Bozzoni

The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

S B Buonomo, A Michienzi, Gian Fabrizio De Angelis, I Bozzoni

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.

Original language	English
Pages (from-to)	993-1002
Number of pages	10
Journal	RNA
Volume	5
Issue number	8
Publication status	Published - Aug 1999

Keywords / Materials (for Non-textual outputs)

Animals
Base Sequence
Cell Line
Cell Nucleus
Chromosomal Proteins, Non-Histone
Cytoplasm
DEAD-box RNA Helicases
Gene Products, rev
Genes, env
Models, Genetic
Molecular Sequence Data
Precipitin Tests
Protein Kinases
RNA Helicases
RNA, Small Nuclear
Time Factors
Transfection
Xenopus
Journal Article
Research Support, Non-U.S. Gov't

Cite this

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title = "The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element",

abstract = "Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.",

keywords = "Animals, Base Sequence, Cell Line, Cell Nucleus, Chromosomal Proteins, Non-Histone, Cytoplasm, DEAD-box RNA Helicases, Gene Products, rev, Genes, env, Models, Genetic, Molecular Sequence Data, Precipitin Tests, Protein Kinases, RNA Helicases, RNA, Small Nuclear, Time Factors, Transfection, Xenopus, Journal Article, Research Support, Non-U.S. Gov't",

author = "Buonomo, {S B} and A Michienzi and {Fabrizio De Angelis}, Gian and I Bozzoni",

year = "1999",

month = aug,

language = "English",

volume = "5",

pages = "993--1002",

journal = "RNA",

issn = "1355-8382",

publisher = "Cold Spring Harbor Laboratory Press",

number = "8",

}

TY - JOUR

T1 - The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

AU - Buonomo, S B

AU - Michienzi, A

AU - Fabrizio De Angelis, Gian

AU - Bozzoni, I

PY - 1999/8

Y1 - 1999/8

N2 - Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.

AB - Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.

KW - Animals

KW - Base Sequence

KW - Cell Line

KW - Cell Nucleus

KW - Chromosomal Proteins, Non-Histone

KW - Cytoplasm

KW - DEAD-box RNA Helicases

KW - Gene Products, rev

KW - Genes, env

KW - Models, Genetic

KW - Molecular Sequence Data

KW - Precipitin Tests

KW - Protein Kinases

KW - RNA Helicases

KW - RNA, Small Nuclear

KW - Time Factors

KW - Transfection

KW - Xenopus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 10445874

SN - 1355-8382

VL - 5

SP - 993

EP - 1002

JO - RNA

JF - RNA

IS - 8

ER -

The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

Abstract

Keywords / Materials (for Non-textual outputs)

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