The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

S B Buonomo, A Michienzi, Gian Fabrizio De Angelis, I Bozzoni

Research output: Contribution to journalArticlepeer-review

Abstract

Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.

Original languageEnglish
Pages (from-to)993-1002
Number of pages10
JournalRNA
Volume5
Issue number8
Publication statusPublished - Aug 1999

Keywords

  • Animals
  • Base Sequence
  • Cell Line
  • Cell Nucleus
  • Chromosomal Proteins, Non-Histone
  • Cytoplasm
  • DEAD-box RNA Helicases
  • Gene Products, rev
  • Genes, env
  • Models, Genetic
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Kinases
  • RNA Helicases
  • RNA, Small Nuclear
  • Time Factors
  • Transfection
  • Xenopus
  • Journal Article
  • Research Support, Non-U.S. Gov't

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