The RNAi machinery regulates growth and development in the filamentous fungus Trichoderma atroviride

Nohemi Carreras-Villaseñor, Edgardo U. Esquivel-Naranjo, J. Manuel Villalobos-Escobedo, Cei Abreu-Goodger, Alfredo Herrera-Estrella*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Summary: The RNAi machinery is generally involved in genome protection in filamentous fungi; however, the physiological role of RNAi has been poorly studied in fungal models. Here, we report that in the filamentous fungus Trichoderma atroviride, the products of the dcr2 and rdr3 genes control reproductive development, because mutations in these genes affect conidiation. In addition, Dcr1 together with Dcr2 control vegetative growth since Δdcr1, Δdcr2 and Δdcr1Δdcr2 present morphological alterations. Whole-genome transcriptional analysis of WT, Δdcr1, Δdcr2 and Δdcr1Δdcr2 show that each Dicer controls different biological processes, such as development or metabolism, which could explain the lack of conidiation in the mutants. Finally, we observed sRNAs that are differentially expressed in the WT and Δdcr2. The expression of some of these sRNAs correlates with the expression of differential transcripts, suggesting that these mRNAs may contain the corresponding targets. Together these data show that in T.atroviride, the RNAi machinery plays a central role in endogenous processes such as development and fitness, beyond controlling genome protection against invasive nucleic acids as reported for other fungi.

Original languageEnglish
Pages (from-to)96-112
Number of pages17
JournalMolecular Microbiology
Issue number1
Early online date15 May 2013
Publication statusPublished - 20 Jun 2013
Externally publishedYes


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