TY - JOUR
T1 - The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice
AU - Meyer Sauteur, Patrick M
AU - de Groot, Ruben C A
AU - Estevão, Silvia C
AU - Hoogenboezem, Theo
AU - de Bruijn, Adrianus C J M
AU - Sluijter, Marcel
AU - de Bruijn, Marjolein J W
AU - De Kleer, Ismé M
AU - van Haperen, Rien
AU - van den Brand, Judith M A
AU - Bogaert, Debby
AU - Fraaij, Pieter L A
AU - Vink, Cornelis
AU - Hendriks, Rudi W
AU - Samsom, Janneke N
AU - Unger, Wendy W J
AU - van Rossum, Annemarie M C
N1 - © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].
PY - 2018/1/15
Y1 - 2018/1/15
N2 - Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. Interestingly, Mp carriage is also detected after infection. While B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. We here show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, while this enabled μMT mice to clear pulmonary Mp infection. This is the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.
AB - Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. Interestingly, Mp carriage is also detected after infection. While B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. We here show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, while this enabled μMT mice to clear pulmonary Mp infection. This is the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.
KW - Journal Article
U2 - 10.1093/infdis/jix559
DO - 10.1093/infdis/jix559
M3 - Article
C2 - 29099932
SN - 0022-1899
VL - 217
JO - The Journal of Infectious Diseases
JF - The Journal of Infectious Diseases
IS - 2
ER -