The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice

Patrick M Meyer Sauteur, Ruben C A de Groot, Silvia C Estevão, Theo Hoogenboezem, Adrianus C J M de Bruijn, Marcel Sluijter, Marjolein J W de Bruijn, Ismé M De Kleer, Rien van Haperen, Judith M A van den Brand, Debby Bogaert, Pieter L A Fraaij, Cornelis Vink, Rudi W Hendriks, Janneke N Samsom, Wendy W J Unger, Annemarie M C van Rossum

Research output: Contribution to journalArticlepeer-review

Abstract

Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. Interestingly, Mp carriage is also detected after infection. While B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. We here show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, while this enabled μMT mice to clear pulmonary Mp infection. This is the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

Original languageEnglish
JournalThe Journal of Infectious Diseases
Volume217
Issue number2
Early online date1 Nov 2017
DOIs
Publication statusPublished - 15 Jan 2018

Keywords

  • Journal Article

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