The small subunit of Rubisco and its potential as an engineering target

Yuwei Mao, Ella Catherall, Aranzazú Díaz-Ramos, George R.L. Greiff, Stavros Azinas, Laura Gunn, Alistair J McCormick

Research output: Contribution to journalReview articlepeer-review


Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) catalyses the first rate-limiting step in CO2 fixation and is responsible for the vast majority of organic carbon present in the biosphere. Rubisco’s function and regulation remain an important research topic and a long standing engineering target to enhance the efficiency of photosynthesis for agriculture and green biotechnology. The most abundant form of Rubisco (Form I) consists of eight large and eight small subunits and is found in all plants, algae, cyanobacteria and most phototrophic and chemolithoautotrophic proteobacteria. Although the active sites of Rubisco are located on the large subunits, expression of the small subunit regulates the size of the Rubisco pool in plants and can influence the overall catalytic efficiency of the Rubisco complex. The small subunit is now receiving increasing attention as a potential engineering target to improve the performance of Rubisco. Here we review our current understanding of the role of the small subunit and our growing capacity to explore its potential to modulate Rubisco catalysis using engineering biology approaches.
Original languageEnglish
Article numbererac309
Number of pages19
JournalJournal of Experimental Botany
Early online date18 Jul 2022
Publication statusPublished - 18 Jul 2022


  • algae
  • carboxylatin
  • oxygenation
  • photosynthesis
  • plants


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