The spindle checkpoint: Assays for the analysis of spindle checkpoint arrest and recovery

Josefin Fernius, Kevin G Hardwick

Research output: Contribution to journalArticlepeer-review

Abstract

The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation by inhibiting anaphase onset until all chromosomes have established stable bipolar attachments. Here we describe a number of protocols that can be used to assay the ability of budding and fission yeast cells to (1) establish and maintain a spindle checkpoint arrest, and (2) segregate chromosomes efficiently upon recovery from mitotic arrest. We focus on experimental detail of the budding yeast protocols, but also point out important differences between budding and fission yeast assays.
Original languageEnglish
Pages (from-to)243-58
Number of pages16
JournalMethods in Molecular Biology
Volume545
DOIs
Publication statusPublished - 2009

Fingerprint Dive into the research topics of 'The spindle checkpoint: Assays for the analysis of spindle checkpoint arrest and recovery'. Together they form a unique fingerprint.

Cite this