The TATGARAT box of the HSV-1ICP27 gene is essential for immediate early expression but not critical for efficient replication in vitro or in vivo

A X Sun, G V Devi-Rao, M K Rice, L W Gary, D C Bloom, R M Sandri-Goldin, P Ghazal, E K Wagner

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We constructed a recombinant virus containing a promoter mutation altering the immediate-early expression of the HSV-1 ICP27 transcript, ICP27DeltaSma, which contains a deletion of the "TATG-ARAT'' and surrounding sequences, but retains the rest of the ICP27 promoter. This mutant does not exhibit immediate-early expression of ICP27 using criteria of expression in the absence of de novo protein synthesis and earliest expression in the kinetic cascade. While transcript abundance at 1 h after infection at 0.1 PFU/cell in mouse embryo fibroblasts was significantly altered compared to infections with wt-rescues, by 4 h after infection these differences were diminished or absent. Consistent with this observation, levels of some critical proteins were reduced in the mutant as compared to rescue infections at the earliest times tested, but were equivalent by 8 - 12 h pi. Further, both single and multi-step virus replication was equivalent with both mutants and rescues. Thus, altering the immediate early kinetics of ICP27 leads to a suboptimal quantitative lag-phase in gene expression but without consequence to replication fitness in vitro. Infections in vivo also revealed the ability of mutant and rescue virus to invade the CNS of mice following footpad injections was equivalent. The nature of the role of immediate-early ICP27 expression is discussed in light of these observations.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalVirus Genes
Volume29
Issue number3
Publication statusPublished - Dec 2004

Keywords / Materials (for Non-textual outputs)

  • HSV-1
  • global transcription analysis
  • ICP27
  • TATGARAT
  • Kinetics
  • SIMPLEX-VIRUS TYPE-1
  • DNA MICROARRAY
  • PROTEIN
  • OCT-1
  • TRANSCRIPTION
  • INFECTION
  • VP16
  • KINETICS
  • COMPLEX

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