@article{35c192d6fcb64dbeb98d062df6a65300,
title = "The transcription factor scleraxis differentially regulates gene expression in tenocytes isolated at different developmental stages",
abstract = "The transcription factor scleraxis (SCX) is expressed throughout tendon development and plays a key role in directing tendon wound healing. However, little is known regarding its role in fetal or young postnatal tendons, stages in development that are known for their enhanced regenerative capabilities. Here we used RNA-sequencing to compare the transcriptome of adult and fetal tenocytes following SCX knockdown. SCX knockdown had a larger effect on gene expression in fetal tenocytes, affecting 477 genes in comparison to the 183 genes affected in adult tenocytes, indicating that scleraxis-dependent processes may differ in these two developmental stages. Gene ontology, network and pathway analysis revealed an overrepresentation of extracellular matrix (ECM) remodelling processes within both comparisons. These included several matrix metalloproteinases, proteoglycans and collagens, some of which were also investigated in SCX knockdown tenocytes from young postnatal foals. Using chromatin immunoprecipitation, we also identified novel genes that SCX differentially interacts with in adult and fetal tenocytes. These results indicate a role for SCX in modulating ECM synthesis and breakdown and provide a useful dataset for further study into SCX gene regulation.",
keywords = "chromatin Immunoprecipitation, gene regulation, knockdown, scleraxis, tendon development, transcriptomics",
author = "Paterson, {Y. Z.} and N. Evans and S. Kan and A. Cribbs and Henson, {F. M.D.} and Guest, {D. J.}",
note = "Funding Information: The authors are grateful to the PetPlan Charitable Trust for funding this work ( S17-419-457 ). YP is funded by a Biotechnology and Biological Sciences Research Council ( BBSRC ) doctoral training partnership PhD studentship. Funding Information: The authors are grateful to Miss Emma Goodfellow for collecting the tissue samples and Ms. Ellen Schofield for advice with the RNA-sequencing analysis. Infographics were created with the Mind the Graph platform (https://mindthegraph.com). The authors are grateful to the PetPlan Charitable Trust for funding this work (S17-419-457). YP is funded by a Biotechnology and Biological Sciences Research Council (BBSRC) doctoral training partnership PhD studentship. Sequence data generated have been submitted to the National Centre for Biotechnology Information Gene Expression Omnibus (NCBI GEO, www.ncbi.nlm.nih.gov/geo) accession number GSE149570. Differential expression analysis and normalized counts are included in Supplementary File 1. Sequence data generated have been submitted to the National Centre for Biotechnology Information Gene Expression Omnibus (NCBI GEO, www.ncbi.nlm.nih.gov/geo) accession number GSE149570. Differential expression analysis and normalized counts are included in Supplementary File 1. None. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = sep,
doi = "10.1016/j.mod.2020.103635",
language = "English",
volume = "163",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
}