The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation

Dorothea Droll, Stuart Archer, Katelyn Fenn, Praveen Delhi, Keith Matthews, Christine Clayton

Research output: Contribution to journalArticlepeer-review

Abstract

Proteins with Pumilio RNA binding domains (Puf proteins) are ubiquitous in eukaryotes. Some Puf proteins bind to the 3'-untranslated regions of mRNAs, acting to repress translation and promote degradation; others are involved in ribosomal RNA maturation. The genome of Trypanosoma brucei encodes eleven Puf proteins whose function cannot be predicted by sequence analysis. We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing.
Original languageEnglish
Pages (from-to)1156-62
Number of pages7
JournalFEBS Letters
Volume584
Issue number6
DOIs
Publication statusPublished - Mar 2010

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