The use of irradiation and fusion gene transfer (IFGT) hybrids to isolate DNA clones from human chromosome region 9q33-q34

F Florian, N Hornigold, D K Griffin, J D Delhanty, L Sefton, C Abbott, C Jones, P N Goodfellow, J Wolfe

Research output: Contribution to journalArticlepeer-review

Abstract

We have generated somatic cell hybrids containing fragments of human chromosome arm 9q by an irradiation and fusion technique. No selection for human material was imposed, but of 23 clones analyzed most contained human DNA sequences and many contained multiple fragments of the human chromosome arm. A hybrid that appears to contain only two small fragments of human DNA from the regions of q33 and q34 has been used as a source from which to clone probes specific to those areas of the chromosome.
Original languageEnglish
Pages (from-to)445-53
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume17
Issue number5
Publication statusPublished - 1991

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cell Line
  • Chromosomes, Human, Pair 9
  • Cloning, Molecular
  • Cricetinae
  • DNA Probes
  • Genetic Markers
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins
  • Transfection

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