The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment

Lewis Timimi; Carmen Figueras-Novoa; Elena Marcassa; Oliver Florey; J Kenneth Baillie; Rupert Beale; Rachel Ulferts

doi:10.1080/15548627.2022.2029233

The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment

Lewis Timimi, Carmen Figueras-Novoa, Elena Marcassa, Oliver Florey, J Kenneth Baillie, Rupert Beale, Rachel Ulferts

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. Consequently, Atg8 family members, especially LC3B, are commonly used as a marker of autophagosomes. However, the Atg8 family of proteins are not found solely attached to double-membrane autophagosomes. In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H+-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes.

Original language	English
Pages (from-to)	707-708
Number of pages	1
Journal	Autophagy
Volume	18
Issue number	3
Early online date	8 Mar 2022
DOIs	https://doi.org/10.1080/15548627.2022.2029233
Publication status	Published - 8 Mar 2022

Keywords / Materials (for Non-textual outputs)

Atg4
Atg8
CASM
SopF
V-ATPase
influenza
lipidation
non-canonical autophagy

Access to Document

10.1080/15548627.2022.2029233

Cite this

@article{d00e0fc3e3e649e48e1ca19e589b6569,

title = "The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment",

abstract = "Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. Consequently, Atg8 family members, especially LC3B, are commonly used as a marker of autophagosomes. However, the Atg8 family of proteins are not found solely attached to double-membrane autophagosomes. In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H+-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes.",

keywords = "Atg4, Atg8, CASM, SopF, V-ATPase, influenza, lipidation, non-canonical autophagy",

author = "Lewis Timimi and Carmen Figueras-Novoa and Elena Marcassa and Oliver Florey and Baillie, {J Kenneth} and Rupert Beale and Rachel Ulferts",

note = "Funding Information: This research was funded in whole, or in part, by the Wellcome Trust [FC011104]. and by the Francis Crick Institute which receives its core funding from Cancer Research UK [FC011104], the UK Medical Research Council [FC011104], and the Wellcome Trust [FC011104]. Publisher Copyright: {\textcopyright} 2022 Informa UK Limited, trading as Taylor & Francis Group.",

year = "2022",

month = mar,

day = "8",

doi = "10.1080/15548627.2022.2029233",

language = "English",

volume = "18",

pages = "707--708",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Landes Bioscience",

number = "3",

}

TY - JOUR

T1 - The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment

AU - Timimi, Lewis

AU - Figueras-Novoa, Carmen

AU - Marcassa, Elena

AU - Florey, Oliver

AU - Baillie, J Kenneth

AU - Beale, Rupert

AU - Ulferts, Rachel

N1 - Funding Information: This research was funded in whole, or in part, by the Wellcome Trust [FC011104]. and by the Francis Crick Institute which receives its core funding from Cancer Research UK [FC011104], the UK Medical Research Council [FC011104], and the Wellcome Trust [FC011104]. Publisher Copyright: © 2022 Informa UK Limited, trading as Taylor & Francis Group.

PY - 2022/3/8

Y1 - 2022/3/8

N2 - Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. Consequently, Atg8 family members, especially LC3B, are commonly used as a marker of autophagosomes. However, the Atg8 family of proteins are not found solely attached to double-membrane autophagosomes. In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H+-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes.

AB - Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. Consequently, Atg8 family members, especially LC3B, are commonly used as a marker of autophagosomes. However, the Atg8 family of proteins are not found solely attached to double-membrane autophagosomes. In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H+-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes.

KW - Atg4

KW - Atg8

KW - CASM

KW - SopF

KW - V-ATPase

KW - influenza

KW - lipidation

KW - non-canonical autophagy

U2 - 10.1080/15548627.2022.2029233

DO - 10.1080/15548627.2022.2029233

M3 - Article

C2 - 35258397

SN - 1554-8627

VL - 18

SP - 707

EP - 708

JO - Autophagy

JF - Autophagy

IS - 3

ER -

The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this