The Wilms' tumor gene WT1 is a common marker of progenitor cells in fetal liver

Keisuke Kanato, Naoki Hosen, Masashi Yanagihara, Naomi Nakagata, Toshiaki Shirakata, Tsutomu Nakazawa, Sumiyuki Nishida, Akihiro Tsuboi, Manabu Kawakami, Tomoki Masuda, Yoshihiro Oka, Yusuke Oji, Annemieke Ijpenberg, Nicholas D Hastie, Haruo Sugiyama

Research output: Contribution to journalArticlepeer-review

Abstract

It is well known that the Wilms' tumor gene WT1 plays an important role in cell proliferation and differentiation, and in organ development. In this study, to examine the role of the WT1 gene in lineage determination, fetal liver cells from LacZ-transgenic mice, in which WT1 expression was marked by the expression of the LacZ gene driven by WT1 promoter, were FACS-sorted according to LacZ expression of high (LacZ(++)) or undetectable (LacZ(-)) levels, which paralleled endogenous WT1 expression levels. LacZ(++) fetal liver cells were enriched by hepatocyte and endothelial progenitor cells. These results indicated that WT1 expression is a common marker of both hepatocyte and endothelial progenitors. These results also implied a role of the WT1 gene in lineage determination.
Original languageEnglish
Pages (from-to)836-43
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number4
DOIs
Publication statusPublished - 28 Jan 2005

Keywords

  • Animals
  • Cells, Cultured
  • Gene Expression Regulation, Developmental
  • Genes, Wilms Tumor
  • Genetic Markers
  • Genetic Testing
  • Hepatocytes
  • Liver
  • Mice
  • Mice, Transgenic
  • Stem Cells
  • WT1 Proteins
  • beta-Galactosidase

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