Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

Christian Mangani, Annamaria Lilienkampf, Marcia Roy, Paul A de Sousa, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

Original languageEnglish
Pages (from-to)1371-5
Number of pages5
JournalBiomaterials science
Issue number10
Publication statusPublished - 15 Oct 2015


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