Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

Christian Mangani; Annamaria Lilienkampf; Marcia Roy; Paul A de Sousa; Mark Bradley

doi:10.1039/c5bm00121h

Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

Christian Mangani, Annamaria Lilienkampf, Marcia Roy, Paul A de Sousa, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

Original language	English
Pages (from-to)	1371-5
Number of pages	5
Journal	Biomaterials science
Volume	3
Issue number	10
DOIs	https://doi.org/10.1039/c5bm00121h
Publication status	Published - 15 Oct 2015

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C5BM00121HAccepted author manuscript, 919 KB

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title = "Thermoresponsive hydrogel maintains the mouse embryonic stem cell {"}na{\"i}ve{"} pluripotency phenotype",

abstract = "A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a {"}na{\"i}ve{"} state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.",

author = "Christian Mangani and Annamaria Lilienkampf and Marcia Roy and {de Sousa}, {Paul A} and Mark Bradley",

year = "2015",

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doi = "10.1039/c5bm00121h",

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AU - Mangani, Christian

AU - Lilienkampf, Annamaria

AU - Roy, Marcia

AU - de Sousa, Paul A

AU - Bradley, Mark

PY - 2015/10/15

Y1 - 2015/10/15

N2 - A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

AB - A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

U2 - 10.1039/c5bm00121h

DO - 10.1039/c5bm00121h

M3 - Article

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EP - 1375

JO - Biomaterials science

JF - Biomaterials science

IS - 10

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Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

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