Thermosensitivity of translation underlies the mammalian nocturnal-diurnal switch

Andrew D Beale, Nina M Rzechorzek, Andrei Mihut, Aiwei Zeng, Nicola J Smyllie, Violetta Pilorz, Rose Richardson, Mads F Bertlesen, Nathan R James, Shaline V Fazal, Zanna Voysey, Jerry Pelletier, Priya Crosby, Sew Y Peak-Chew, Madeline A Lancaster, Roelof A Hut, John O'Neill

Research output: Working paperPreprint

Abstract / Description of output

Early mammals were nocturnal until the Cretaceous-Paleogene extinction facilitated their rapid expansion into daytime niches. Diurnality subsequently evolved multiple times, independently, but the mechanisms facilitating this switch are unknown. We found that physiological daily temperature shifts oppositely affect circadian clock rhythms in nocturnal versus diurnal mammals. This occurs through a cell-intrinsic signal inverter, mediated by global differences in protein phosphorylation, and effected at the level of bulk protein synthesis rates, with diurnal translation rate being less thermosensitive than nocturnal. Perturbations that reduce translational initiation or mTOR activity are sufficient to trigger the nocturnal-to-diurnal switch at the cellular, tissue, and organismal scale. Our results suggest a convergent selection pressure to attain diurnality by reducing the effect of temperature-dependent changes in protein synthesis on circadian clocks.
Original languageEnglish
Number of pages41
Publication statusPublished - 22 Jun 2023


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