Three-dimensional imaging of Drosophila melanogaster

Leeanne McGurk, Harris Morrison, Liam P Keegan, James Sharpe, Mary A O'Connell

Research output: Contribution to journalArticlepeer-review


The major hindrance to imaging the intact adult Drosophila is that the dark exoskeleton makes it impossible to image through the cuticle. We have overcome this obstacle and describe a method whereby the internal organs of adult Drosophila can be imaged in 3D by bleaching and clearing the adult and then imaging using a technique called optical projection tomography (OPT). The data is displayed as 2D optical sections and also in 3D to provide detail on the shape and structure of the adult anatomy.
Original languageEnglish
Pages (from-to)e834
JournalPLoS ONE
Issue number9
Publication statusPublished - 2007


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