Three-dimensional imaging of Drosophila melanogaster

Leeanne McGurk; Harris Morrison; Liam P Keegan; James Sharpe; Mary A O'Connell

doi:10.1371/journal.pone.0000834

Three-dimensional imaging of Drosophila melanogaster

Leeanne McGurk, Harris Morrison, Liam P Keegan, James Sharpe, Mary A O'Connell

Deanery of Molecular, Genetic and Population Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The major hindrance to imaging the intact adult Drosophila is that the dark exoskeleton makes it impossible to image through the cuticle. We have overcome this obstacle and describe a method whereby the internal organs of adult Drosophila can be imaged in 3D by bleaching and clearing the adult and then imaging using a technique called optical projection tomography (OPT). The data is displayed as 2D optical sections and also in 3D to provide detail on the shape and structure of the adult anatomy.

Original language	English
Pages (from-to)	e834
Journal	PLoS ONE
Volume	2
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0000834
Publication status	Published - 2007

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10.1371/journal.pone.0000834

Three-dimensional imaging of Drosophila melanogaster
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http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0000834

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Three-dimensional imaging of Drosophila melanogaster

Abstract

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