Abstract / Description of output
Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microﬂuidic mixing device. The resulting experimental data are compared with computational ﬂuid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing proﬁle to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of conﬂuence. Following experimental and CFD optimization, mixing times down to 1.3±0.4 ms were achieved with the single-layer microﬂuidic device.