Three-Dimensional molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging

T. Robinson; Prashant Valluri; H. B. Manning; D. M. Owen; I. Munro; C. B. Talbot; C. Dunsby; J. F. Eccleston; G. S. Baldwin; M. A. A. Neil; A. J. de Mello; P. M. W. French

doi:10.1364/OL.33.001887

Three-Dimensional molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging

T. Robinson, Prashant Valluri, H. B. Manning, D. M. Owen, I. Munro, C. B. Talbot, C. Dunsby, J. F. Eccleston, G. S. Baldwin, M. A. A. Neil, A. J. de Mello, P. M. W. French

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microﬂuidic mixing device. The resulting experimental data are compared with computational ﬂuid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing proﬁle to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of conﬂuence. Following experimental and CFD optimization, mixing times down to 1.3±0.4 ms were achieved with the single-layer microﬂuidic device.

Original language	English
Pages (from-to)	1887-1889
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	16
DOIs	https://doi.org/10.1364/OL.33.001887
Publication status	Published - Jan 2008

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title = "Three-Dimensional molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging",

abstract = "Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microﬂuidic mixing device. The resulting experimental data are compared with computational ﬂuid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing proﬁle to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of conﬂuence. Following experimental and CFD optimization, mixing times down to 1.3±0.4 ms were achieved with the single-layer microﬂuidic device.",

author = "T. Robinson and Prashant Valluri and Manning, {H. B.} and Owen, {D. M.} and I. Munro and Talbot, {C. B.} and C. Dunsby and Eccleston, {J. F.} and Baldwin, {G. S.} and Neil, {M. A. A.} and Mello, {A. J. de} and French, {P. M. W.}",

year = "2008",

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doi = "10.1364/OL.33.001887",

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T1 - Three-Dimensional molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging

AU - Robinson, T.

AU - Valluri, Prashant

AU - Manning, H. B.

AU - Owen, D. M.

AU - Munro, I.

AU - Talbot, C. B.

AU - Dunsby, C.

AU - Eccleston, J. F.

AU - Baldwin, G. S.

AU - Neil, M. A. A.

AU - Mello, A. J. de

AU - French, P. M. W.

PY - 2008/1

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AB - Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microﬂuidic mixing device. The resulting experimental data are compared with computational ﬂuid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing proﬁle to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of conﬂuence. Following experimental and CFD optimization, mixing times down to 1.3±0.4 ms were achieved with the single-layer microﬂuidic device.

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JF - Optics Letters

IS - 16

ER -

Three-Dimensional molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging

Abstract

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