Three-dimensional reconstruction of tetraploid<->diploid chimaeric mouse blastocysts

C A Everett, M H Stark, J D West, D Davidson, R A Baldock

Research output: Contribution to journalArticlepeer-review

Abstract

Studies of tetraploiddiploid (4n2n) mouse chimaeras have demonstrated unequal contributions of 4n cells to different tissues of the midgestation conceptus. Such a pattern has also been reported in chimaeras as early as E3.5d, which show an enhanced contribution of 4n cells to the mural trophectoderm (Everett & West, 1996). In this study, sectioned 4n2n and 2n2n control chimaeric blastocysts were digitised and reconstructed in 3 dimensions (3-D). The 3-D images revealed only limited mixing of cells from the 2 contributing embryos of individual blastocysts in both chimaera groups. Consequently, the distribution pattern of the 2 cell types was dependent on the spatial relationship between the orientation of the blastocyst and the boundary between the 2 clusters of cells. The distribution patterns observed were not strikingly different for 4n2n and 2n2n chimaeras, each showing some transgenic positive cell contribution in all 3 identifiable developmental lineages. It was notable, however, that in all 4n2n blastocysts at least some 4n cells were located adjacent to the blastocyst cavity. Such a consistent pattern was not evident in 2n2n chimaeras. This study has demonstrated the value of 3-D reconstructions for the analysis of spatial relationships of 2 cell populations in chimaeric mouse blastocysts.
Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalJournal of Anatomy
Volume196
Issue number3
DOIs
Publication statusPublished - Apr 2000

Keywords

  • Animals
  • Polyploidy
  • Chimera
  • Cell Lineage
  • Blastocyst
  • Gestational Age
  • Microscopy, Electron
  • Mice
  • Ploidies
  • Image Processing, Computer-Assisted
  • Diploidy

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