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Abstract / Description of output
Quantitative polymerase chain reaction (qPCR) is a fundamental technique in molecular biology to detect and quantify DNA and RNA. Here we present the tidyqpcr software package for user-friendly qPCR analysis using the tidyverse suite of R packages. tidyqpcr offers a consistent user interface and structure for qPCR analysis, within the tidyverse paradigm of spreadsheet-like rectangular data frames and generic functions that build up complex analyses in a series of simple steps. tidyqpcr focuses on experimental design in microwell plates, and relative quantification using changes in quantification cycle (∆Cq). Overall, tidyqpcr empowers scientists to conduct reproducible, flexible, and best-practice compliant quantitative PCR analysis.
|Publisher||ELIFE SCIENCES PUBLICATIONS LTD|
|Publication status||Published - 27 Jun 2022|
|Name||Journal of Open Source Software|
Keywords / Materials (for Non-textual outputs)
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- 1 Finished
19/01/18 → 18/01/23