Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

Day-Uei Li; Simon Ameer-Beg; Jochen Arlt; David Tyndall; Richard Walker; Daniel Matthews; Viput Visitkul; J. Richardson; Robert Henderson

doi:10.3390/s120505650

Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

Day-Uei Li, Simon Ameer-Beg, Jochen Arlt, David Tyndall, Richard Walker, Daniel Matthews, Viput Visitkul, J. Richardson, Robert Henderson

Research output: Contribution to journal › Article › peer-review

Abstract

We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.

Original language	English
Pages (from-to)	5650-5659
Number of pages	10
Journal	Sensors
Volume	12
Issue number	5
DOIs	https://doi.org/10.3390/s120505650
Publication status	Published - 2 May 2012

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title = "Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays",

abstract = "We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast. ",

author = "Day-Uei Li and Simon Ameer-Beg and Jochen Arlt and David Tyndall and Richard Walker and Daniel Matthews and Viput Visitkul and J. Richardson and Robert Henderson",

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AU - Li, Day-Uei

AU - Ameer-Beg, Simon

AU - Arlt, Jochen

AU - Tyndall, David

AU - Walker, Richard

AU - Matthews, Daniel

AU - Visitkul, Viput

AU - Richardson, J.

AU - Henderson, Robert

PY - 2012/5/2

Y1 - 2012/5/2

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AB - We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.

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DO - 10.3390/s120505650

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JO - Sensors

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Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

Abstract

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