Projects per year
Abstract
The plant‐specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post‐translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time‐specific manner, using proteomics on Arabidopsis plants expressing epitope‐tagged GI. We detected previously identified (in)direct interactors of GI, as well as proteins implicated in protein folding, or degradation, and a previously uncharacterized transcription factor, CYCLING DOF FACTOR6 (CDF6). We verified CDF6's direct interaction with GI, and ZEITLUPE/FLAVIN‐BINDING, KELCH REPEAT, F‐BOX 1/LIGHT KELCH PROTEIN 2 proteins, and demonstrated its involvement in photoperiodic flowering. Extending interaction proteomics to time series provides a data resource of candidate protein targets for GI's post‐translational control.
Original language | English |
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Number of pages | 20 |
Journal | FEBS Letters |
Volume | 593 |
Issue number | 3 |
Early online date | 7 Dec 2018 |
DOIs | |
Publication status | Published - Feb 2019 |
Keywords
- Circadian rhythms
- Flowering time
- Affinity purification
- Quantitative mass spectrometry
- Arabidopsis thaliana
Fingerprint
Dive into the research topics of 'Time-resolved Interaction Proteomics of the GIGANTEA Protein Under Diurnal Cycles in Arabidopsis'. Together they form a unique fingerprint.Projects
- 4 Finished
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14 ERA-CAPS PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops
Halliday, K., Krahmer, J. & Romanowski, A.
1/10/15 → 30/03/19
Project: Research
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Does an Ancient Circadian Clock control transcriptional rhythms using a non transcriptional oscillator
Millar, A. & Le Bihan, T.
1/10/12 → 31/01/16
Project: Research
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R42038 Wellcome Trust Four Year PhD Studentship Ms Johanna Krahmer
Beggs, J.
1/10/11 → 30/09/15
Project: Research
Profiles
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Karen Halliday
- School of Biological Sciences - Personal Chair of Systems Physiology
- Global Academy of Agriculture and Food Systems
Person: Academic: Research Active