Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy

Dario Angelone; Sanathana Konugolu Venkata Sekar; Walter Messina; Andrew Matheson; Ahmet T. Erdogan; Robert K. Henderson; Ray Burke; Stefan Andersson-Engels

doi:10.1117/12.2648340

Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy

Dario Angelone^*, Sanathana Konugolu Venkata Sekar, Walter Messina, Andrew Matheson, Ahmet T. Erdogan, Robert K. Henderson, Ray Burke, Stefan Andersson-Engels

^*Corresponding author for this work

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract / Description of output

We propose a handheld single photon avalanche diode (SPAD) micro-camera probe for wide-field in-vivo fluorescence lifetime imaging (FLIm) applications. The presented probe includes a novel 3D stacked 1.4 mm × 1.4 mm SPAD array, an integrated excitation light source, and imaging optics. The spatial and temporal performance of the integrated system was characterised using a USAF test target and range of fluorescence lifetime beads.

Original language	English
Title of host publication	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI
Editors	Caroline Boudoux, James W. Tunnell
Publisher	SPIE
Volume	12368
ISBN (Electronic)	9781510658417
DOIs	https://doi.org/10.1117/12.2648340
Publication status	Published - 6 Mar 2023
Event	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI 2023 - San Francisco, United States Duration: 28 Jan 2023 → 29 Jan 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12368
ISSN (Print)	1605-7422

Conference

Conference	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI 2023
Country/Territory	United States
City	San Francisco
Period	28/01/23 → 29/01/23

Keywords / Materials (for Non-textual outputs)

FLIm
Fluorescence lifetime imaging
Micro-camera
SPAD

Access to Document

10.1117/12.2648340

Cite this

Angelone, D., Konugolu Venkata Sekar, S., Messina, W., Matheson, A., Erdogan, A. T., Henderson, R. K., Burke, R., & Andersson-Engels, S. (2023). Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy. In C. Boudoux, & J. W. Tunnell (Eds.), Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI (Vol. 12368). Article 1236807 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12368). SPIE. https://doi.org/10.1117/12.2648340

Angelone, Dario ; Konugolu Venkata Sekar, Sanathana ; Messina, Walter et al. / Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy. Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI. editor / Caroline Boudoux ; James W. Tunnell. Vol. 12368 SPIE, 2023. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{45f4e6fc9cb5401994eb7714a12cc86c,

title = "Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy",

abstract = "We propose a handheld single photon avalanche diode (SPAD) micro-camera probe for wide-field in-vivo fluorescence lifetime imaging (FLIm) applications. The presented probe includes a novel 3D stacked 1.4 mm × 1.4 mm SPAD array, an integrated excitation light source, and imaging optics. The spatial and temporal performance of the integrated system was characterised using a USAF test target and range of fluorescence lifetime beads.",

keywords = "FLIm, Fluorescence lifetime imaging, Micro-camera, SPAD",

author = "Dario Angelone and {Konugolu Venkata Sekar}, Sanathana and Walter Messina and Andrew Matheson and Erdogan, {Ahmet T.} and Henderson, {Robert K.} and Ray Burke and Stefan Andersson-Engels",

note = "Funding Information: We gratefully acknowledge funding from the EU H2020 MSCA-ITN project “Photonics for Healthcare: mul-tiscale cancer diagnosis and therapy” (PHAST-ETN), grant agreement no. 86018; Science Foundation Ireland (SFI/15/RP/2828 and SFI-12/RC/2276 P2); and the PROTEUS project (http://proteus.ac.uk), Grant no: EP/K03197X/1. Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI 2023 ; Conference date: 28-01-2023 Through 29-01-2023",

year = "2023",

month = mar,

day = "6",

doi = "10.1117/12.2648340",

language = "English",

volume = "12368",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Caroline Boudoux and Tunnell, {James W.}",

booktitle = "Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI",

address = "United States",

}

Angelone, D, Konugolu Venkata Sekar, S, Messina, W, Matheson, A, Erdogan, AT , Henderson, RK, Burke, R & Andersson-Engels, S 2023, Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy. in C Boudoux & JW Tunnell (eds), Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI. vol. 12368, 1236807, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12368, SPIE, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI 2023, San Francisco, United States, 28/01/23. https://doi.org/10.1117/12.2648340

Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy. / Angelone, Dario; Konugolu Venkata Sekar, Sanathana; Messina, Walter et al.
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI. ed. / Caroline Boudoux; James W. Tunnell. Vol. 12368 SPIE, 2023. 1236807 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12368).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy

AU - Angelone, Dario

AU - Konugolu Venkata Sekar, Sanathana

AU - Messina, Walter

AU - Matheson, Andrew

AU - Erdogan, Ahmet T.

AU - Henderson, Robert K.

AU - Burke, Ray

AU - Andersson-Engels, Stefan

N1 - Funding Information: We gratefully acknowledge funding from the EU H2020 MSCA-ITN project “Photonics for Healthcare: mul-tiscale cancer diagnosis and therapy” (PHAST-ETN), grant agreement no. 86018; Science Foundation Ireland (SFI/15/RP/2828 and SFI-12/RC/2276 P2); and the PROTEUS project (http://proteus.ac.uk), Grant no: EP/K03197X/1. Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2023/3/6

Y1 - 2023/3/6

N2 - We propose a handheld single photon avalanche diode (SPAD) micro-camera probe for wide-field in-vivo fluorescence lifetime imaging (FLIm) applications. The presented probe includes a novel 3D stacked 1.4 mm × 1.4 mm SPAD array, an integrated excitation light source, and imaging optics. The spatial and temporal performance of the integrated system was characterised using a USAF test target and range of fluorescence lifetime beads.

AB - We propose a handheld single photon avalanche diode (SPAD) micro-camera probe for wide-field in-vivo fluorescence lifetime imaging (FLIm) applications. The presented probe includes a novel 3D stacked 1.4 mm × 1.4 mm SPAD array, an integrated excitation light source, and imaging optics. The spatial and temporal performance of the integrated system was characterised using a USAF test target and range of fluorescence lifetime beads.

KW - FLIm

KW - Fluorescence lifetime imaging

KW - Micro-camera

KW - SPAD

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U2 - 10.1117/12.2648340

DO - 10.1117/12.2648340

M3 - Conference contribution

AN - SCOPUS:85152765546

VL - 12368

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI

A2 - Boudoux, Caroline

A2 - Tunnell, James W.

PB - SPIE

T2 - Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI 2023

Y2 - 28 January 2023 through 29 January 2023

ER -

Angelone D, Konugolu Venkata Sekar S, Messina W, Matheson A, Erdogan AT , Henderson RK et al. Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy. In Boudoux C, Tunnell JW, editors, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI. Vol. 12368. SPIE. 2023. 1236807. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2648340

Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy

Abstract / Description of output

Publication series

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging

Cite this

Time resolved SPAD micro-camera probe for wide-field FLIm in microendoscopy

Abstract / Description of output

Publication series

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging

Cite this