TY - JOUR
T1 - TISSUE ENGINEERING FOR TOTAL MENISCAL SUBSTITUTION. Animal study in sheep model
T2 - results at 12 months
AU - Kon, Elizaveta
AU - Filardo, Giuseppe
AU - Tschon, Matilde
AU - Fini, Milena
AU - Giavaresi, Gianluca
AU - Marchesini Reggiani, Leonardo
AU - Chiari, Catharina
AU - Nehrer, Stefan
AU - Martin, Ivan
AU - Salter, Donald
AU - Ambrosio, Luigi
AU - Marcacci, Maurilio
PY - 2012
Y1 - 2012
N2 - The aim of the study was to investigate the use of a hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Eighteen skeletally mature sheep were treated. The animals were divided in three groups: cell-free scaffold, scaffold seeded with autologous chondrocytes and meniscectomy alone. The implant was sutured to the capsule and to the meniscal ligament. At 12 months gross assessment, histology and histomorphometry were used to assess meniscus implant, knee joint and osteoarthritis development. All implants showed excellent capsular ingrowth at the periphery. Implant gross assessment showed significant differences between cell-seeded and cell-free groups (p=0.011). The histological analysis indicated a cellular colonization throughout the implanted constructs. Avascular cartilaginous tissue formation was significantly more frequent in the cell-seeded constructs. Joint gross assessment showed that sheep treated with scaffold implantation achieved a significant higher score than those who underwent meniscectomy (p
AB - The aim of the study was to investigate the use of a hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Eighteen skeletally mature sheep were treated. The animals were divided in three groups: cell-free scaffold, scaffold seeded with autologous chondrocytes and meniscectomy alone. The implant was sutured to the capsule and to the meniscal ligament. At 12 months gross assessment, histology and histomorphometry were used to assess meniscus implant, knee joint and osteoarthritis development. All implants showed excellent capsular ingrowth at the periphery. Implant gross assessment showed significant differences between cell-seeded and cell-free groups (p=0.011). The histological analysis indicated a cellular colonization throughout the implanted constructs. Avascular cartilaginous tissue formation was significantly more frequent in the cell-seeded constructs. Joint gross assessment showed that sheep treated with scaffold implantation achieved a significant higher score than those who underwent meniscectomy (p
U2 - 10.1089/ten.TEA.2011.0572
DO - 10.1089/ten.TEA.2011.0572
M3 - Article
C2 - 22500654
SN - 1937-335X
JO - Tissue Engineering Part A
JF - Tissue Engineering Part A
ER -