Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons

Morgane Louessard, Alexandre Lacroix, Magalie Martineau, Gregoire Mondielli, Axel Montagne, Flavie Lesept, Bertrand Lambolez, Bruno Cauli, Jean-Pierre Mothet, Denis Vivien, Eric Maubert

Research output: Contribution to journalArticlepeer-review


Although the extracellular serine protease tissue plasminogen activator (tPA) is involved in pathophysiological processes such as learning and memory, anxiety, epilepsy, stroke, and Alzheimer's disease, information about its regional, cellular, and subcellular distribution in vivo is lacking. In the present study, we observed, in healthy mice and rats, the presence of tPA in endothelial cells, oligodendrocytes, mastocytes, and ependymocytes, but not in pericytes, microglial cells, and astrocytes. Moreover, blockage of the axo-dendritic transport unmasked tPA expression in neurons of cortical and hippocampal areas. Interestingly, combined electrophysiological recordings, single-cell reverse transcription polymerase chain reaction (RT-PCR), and immunohistological analyses revealed that the presence of tPA is restricted to subsets of excitatory pyramidal glutamatergic neurons. We further evidenced that tPA is stored in synaptobrevin-2-positive glutamatergic synaptic vesicles. Based on all these data, we propose the existence of tPA-ergic neurons in the mature brain.

Original languageEnglish
Pages (from-to)5000-12
Number of pages13
JournalMolecular Neurobiology
Issue number7
Publication statusPublished - Sep 2016


  • Animals
  • Cells, Cultured
  • Excitatory Postsynaptic Potentials/physiology
  • Gene Expression
  • Glutamic Acid/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Organ Culture Techniques
  • Pyramidal Cells/metabolism
  • Rats
  • Rats, Wistar
  • Synaptic Vesicles/metabolism
  • Tissue Plasminogen Activator/biosynthesis


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