Tissue-specific and expression of porcine growth hormone gene in BAC transgenic mice

Jia Tong, Simon G Lillico, Ming Jun Bi, Tong Qing, Xiao Fang Liu, Yuanyuan Wang, Min Zheng, Meili Wang, Yun Ping Dai, C Bruce A Whitelaw, Ning Li

Research output: Contribution to journalArticlepeer-review

Abstract

One of the primary goals of traditional livestock breeding is to improve growth rate and optimise body size. Growth rate can be significantly increased by integrating a growth hormone (GH) transgene under the control of a ubiquitous promoter, but while such animals do demonstrate increased growth there are also serious deleterious side-effects to the animals health. Here we report the generation and initial characterization of transgenic mice that carried a porcine BAC encoding the porcine GH gene. We show that GH expression is restricted specifically to the pituitary, is associated with elevated IGF-1 levels, and results in growth enhancement. No negative effects to the health of the transgenic animals were detected. This initial characterisation supports the use of BAC pGH transgene in livestock studies.
Original languageEnglish
Pages (from-to)933-8
Number of pages6
JournalTransgenic Research
Volume20
Issue number4
DOIs
Publication statusPublished - Aug 2011

Keywords

  • Animals
  • Chromosomes, Artificial, Bacterial
  • Female
  • Gene Expression
  • Growth Hormone
  • Male
  • Mice
  • Mice, Transgenic
  • Pituitary Gland
  • Swine
  • Tissue Distribution

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